To search for stocks, browse the categories below, or use the Simple Stock Search , but we recommend using the Advanced Stock Search (for identifying stocks) or Advanced Symbol Search (for identifying particular components like alleles, insertions, aberrations) for more refined search capabilities.
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Aberrations Attached-X stocks A complete list of attached-X stocks at BDSC. Compound autosomes Stocks carrying compound autosomes (use to detect primary non-disjunction in females). Translocations An overview of translocations with lists of stocks. Transpositions An overview of transpositions with lists of stocks. Variegating rearrangements Stocks carrying chromosomal rearrangements that result in variegated gene expression.
Balancers Balancers All single-balancer stocks plus selected double-balancer stocks at Bloomington, the definitions of balancer symbols used in genotypes, and the cytological order of balancer rearrangements.
Binary expression systems (GAL4/UAS, lexA/lexAop, QF/QS, TALE/VAS system etc.) All GAL4 stocks This main GAL4 page lists all GAL4 subsets at Bloomington. All GAL80 stocks GAL80 insertions at Bloomington. All lexA/lexAop stocks All lexA and lexAop stocks at Bloomington. All Q system stocks All QUAS, QF and QS insertions at Bloomington. All UAS stocks This main UAS page lists all UAS subsets at Bloomington. Alternative systems tet, cym, pip, van and ttg drivers and responders. HACK tools Converting GAL4 into QF2, GAL80 or split-GAL4 effectors. InSITE GAL4 All InSITE insertions at Bloomington. Split transcriptional activators 'Split' transcriptional activators based on the DNA binding or activation domains of regulators like GAL4, lexA, QF and p65. To further refine patterns, see the Killer Zipper page . TALE/VAS system Expresses VAS-transgene under the control of promoter-TALE. UAS & lexAop - Pfeiffer set UAS & lexAop-regulated transgenes with varying numbers of repeats driving fluors or GAL80 from Pfeiffer et al., 2010 .
Chemically induced mutations EMS-induced X lethals X chromosome lethals induced with low concentrations of EMS, from the Bellen lab.
Clonal/lineage analysis Overview Brief descriptions and links to pages for lineage and clonal analysis system DMARCM DMARCM is similar to MARCM but requires two recombination events for expression. G-TRACE and i-TRACE Lines for cell lineage analysis Germline clonal analysis Stocks for assessing gene function in the female germ line by clonal analysis using "ovo[D]" stocks. IntMEMOIR An array of 10 unique invertible barcodes for cell lineage analysis Lineage filtering Restricted expression through patterned recombination. MAGIC Mosaic Analysis by gRNA-induced Crossing-over. MARCM MARCM lines for mosaic analysis from the Luo lab. MARCM - Twin spot Stocks for marking both sister clones following a mitotic recombination event. Mitotic recombination stocks Stocks for detecting induced or spontaneous mitotic recombination via cuticular phenotypes. MultiColor FlpOut Lines for cell lineage analysis. Photoreceptor clones Stocks for making marked photoreceptor clones. Raeppli Lines for cell lineage analysis. SPARC Restricted expression (sparsening) through recombination. TIE-DYE Line for cell lineage analysis.
Deficiencies All Deficiencies Links to all deficiencies available from Bloomington. BSC & Exelixis Deficiencies Molecularly defined deficiencies from BDSC and Exelixis, Inc. , and stocks for making new deficiencies from Exelixis insertions. Deficiency kit The subset of deficiencies that make up the Bloomington Deficiency Kit . Deficiency subset - Zinn A subset of deficiencies defined by the Zinn lab for screening homozygous embryos. DrosDel Deficiencies Molecularly defined deficiencies from the DrosDel Project and stocks used to create FLP recombinase-mediated deletions between insertions of the FRT-bearing constructs P{RS5} and P{RS3}.
Duplications All Duplications Links to all available molecularly and cytologically defined duplications. Bloomington Duplication Project The Bloomington Duplication Project generated coverage of 97-98% of the X chromosome with Y-linked duplications. Duplication Consortium Project X chromosome duplications placed in an attP site on Chromosome 3. They are generally five to twenty genes in size. X Chromosome Duplication Kit A subset of duplications representing the X chromosome duplication kit. 2nd Chromosome Duplication Kit A subset of duplications representing the 2nd chromosome duplication kit. 3rd Chromosome Duplication Kit A subset of duplications representing the 3rd chromosome duplication kit.
Fluorescent protein, luciferase, lacZ, antibody and other reporters/tools Complete list of fluorescent protein stocks Stocks expressing GFP, CFP, RFP, YFP, DsRed and other fluors, including stocks with UAS-fluor transgenes. Antibody fragments Stocks expressing antibody fragments. Calcium sensors Fluorescent or transcription -based reporters of intracellular Ca[2+] for reporting calcium-dependent neuronal activity.Cell cycle fluorescent indicators RGB-cct and Fly-FUCCI Chemical tags Lines carrying CLIP, SNAP, Halo and TMP tags for labeling subcellular compartments. deGradFP Stocks for eliminating GFP-tagged proteins. FRET biosensors Fluorescence resonance energy transfer biosensors GFP-lacI and lacO Target GFP to specific chromosomal sites in live cells. iGFPi Stocks for RNAi of mRNAs with GFP sequences by the in vivo GFP interference (iGFPi) method. GrabFP Stock expressing anti-GFP antibodies for localization of GFP and GFP-tagged proteins to particular cellular compartments. lacZ reporters Transgenes expressing lacZ under the control of gene regulatory sequences or lacZ fusion protein. Luciferase Transgenes expressing luciferase (plain or split). Optogenetic tools Optogenetic actuators Photocontrollable FP OptoTrap Photoreceptor clones Stocks for making marked photoreceptor clones. proPO staining An alternative to using UAS-lacZ with X-gal. Protein regulation by ubiquitination and proteasomal degradation Auxin-inducible protein degradation , Ubiquitination tags .Protein-Trap insertions FlyTrap , MiMIC-RMCE and Mi{Hto} fluorescent protein trap insertions.Redox sensors Stocks carrying a redox-sensitive GFP for determining redox conditions in mitochondria or the cytoplasm. RNA isolation stocks Stocks for isolating RNA from specific cells. Sexing embryos Distinguishing female from male embryos with GFP. smGdP scaffolds with epitope tags Lines carrying Flag, HA, Myc, OLLAS etc tags in darkened spaghetti monster green fluorescent proteins (smGdP). SPARC Restricted expression (sparsening) of GCaMP and more through recombination. SPARK Phase-separation based GFP reporter of kinase activity. STaR Lines for specifically marking pre- and/or post-synaptic terminals. Transsynaptic tracing Trans-Tango and BAcTrace lines for labeling or manipulating pre- or post-synaptic neurons. Virus Detection Stocks for detection of viral infection. Voltron (also see voltage sensors )Stocks carrying Voltron, a chemigenetic voltage sensor
FLP/FRT and other recombinases (B2, B3, cre, KD and RSR) FLP/FRT and other recombinases lists of FLP/FRT and other recombinase systems B2, B3, cre, KD and RSR.
Genome editing stocks (CRISPR (Cas9, gRNA), homologous recombination, Zn finger nucleases) CRISPR - Cas nucleases Cas9, Cas12a and tracrRNA stocks .CRISPR - DNA base editors Cas9-based editors for making point mutations CRISPR - gRNA guide RNA stocks for site-specific mutagenesis or gene activation. CRISPR - P{white-eraser} for changing a mini-w+ marker to 3xP3-RFP CRISPR - UAS-Cas9 & GAL4 TRiP Toolbox set - stocks with both UAS-Cas9 and a GAL4 driver for gene activation or knockout. Ends-in and ends-out gene targeting I-Scel-directed gene targeting stocks for homologous recombination. Zinc-finger nuclease Stocks for creating double-stranded breaks in y or ry using Zn-finger nucleases.
Human disease models Human Disease Models Stocks that can be used to study a variety of human diseases as well as human health-related subjects such as metabolism and genes and pathways associated with these processes. Orthologous genes in Drosophila and human Lists of orthologous genes retrieved from Ensembl BioMart and FlyBase
Insertions and tools for insertion manipulation (phiC31, RMCE, transposases, etc) Insertion collections All transposon insertions All insertion collections at Bloomington, sorted by location. Exelixis insertions P{} and PBac{} insertions generated by Exelixis, sorted by insertion site sequence coordinates. Gene Disruption Project insertions All insertions at Bloomington that were accessioned through the Berkeley Drosophila Genome Project or the Gene Disruption Project's insertion selection pipeline. Hostile takeover insertions All Mi{Hto} insertions at Bloomington. MiMIC and CRIMIC insertions All Mi{MIC} and TI{CRIMIC} insertions. These contain two attP sites that can be used for RMCE. InSITE GAL4 insertions All InSITE insertions. These contain a single attP site that can be used for RMCE. lacZ enhancer trap lines Stocks carrying P{lacW} , P{PZ} , P{lArB} , P{lwB} , P{A92} and others expressing lacZ.Minos insertions Lists of Mi{} -based insertions, sorted by chromosome. piggyBac insertions Lists of PBac{}-based insertions sorted by chromosome .Potential misexpression insertions All P{EP}, P{EPg}, P{EPgy2}, P{Mae-UAS.6.11}, P{XP} and PBac{WH} insertions at Bloomington. Protein-trap insertions FlyTrap , MiMIC-RMCE and Mi{Hto} fluorescent protein trap insertions.
Tools for manipulating insertions Imprecise excisions Stocks for increasing frequency and size of imprecise deletions using blm mutations HACK tools Lines for converting GAL4 into QF2, GAL80 or split-GAL4 effectors. phiC31 components All stocks with components of the phiC31 integration system. RMCE donors for InSITEs Lines carrying donor cassettes for Recombination-Mediated Cassette Exchange with InSITE insertions. RMCE donors for MiMICs Lines carrying donor cassettes for Recombination-Mediated Cassette Exchange with MiMIC insertions. Transposable element mutagenesis Selected stocks for P, H, PBac and Mi transposable element mutagenesis.
Mapping Mapping stocks A selection of stocks for mapping, including SNP lines. Baylor P{} mapping kits Predefined sets of P-insertion lines for mapping point mutations (Zhai et al., 2003 ).
Multiplex components Multiplex components Transgenes carrying multiple independently regulated fragments on the same construct.
RNAi/miRNA All RNAi stocks This main RNAi page lists all RNAi subsets at Bloomington, sorted by gene. iGFPi Stocks for RNAi of mRNAs with GFP sequences by the in vivo GFP interference (iGFPi) method. mir gene mutations mir gene knockouts, most are from Steve Cohen and colleagues. mir sponge lines Lines carrying UAS constructs for knocking down microRNA expression.
Teaching stocks Dr Phil's flightless flies Use Drosophila to demonstrate segregation, sex chromosome linkage etc with flightless flies Educational resources Links to information on how to work with flies, phenotypic marker images and other educational resources. Early development lab Y. Hiromi stocks for labs on early development. Isozymes lab Stocks containing isoenzyme alleles associated with different gel mobility. Mapping lab D. Dean genetics "fly lab" for mapping an unknown mutation Natural selection lab M. Noor stocks for a lab on natural selection and hitchhiking. Optogenetics lab Use Drosophila to demonstrate optogenetic tools. Eye color trait lab Uses bw and st to illustrate a trait due to two recessive alleles.
Wild-type and isogenic lines All wild-type stocks All wild-type stocks at Bloomington. The reference genome strain is 2057 . Genetic reference panel (DGRP) Sequenced inbred wild-type lines from Raleigh, NC population (Mackay, Richards & Gibbs ). Drosophila synthetic population resource (DSPR) Genotyped Drosophila Synthetic Population Resource strains. Isogenized chromosomes Selected stocks with one or more isogenic chromosomes. Malawi lines Sequenced chromosomes extracted from a Malawi population in laboratory stock backgrounds, from the Langley lab. Sleep inbred panel (SIP) Stocks established from s equenced DGRP strains selected for long and short night-time sleep
Y chromosomes with markers or transgenes
More stocks... Cell line generation Stocks for generating cell lines using activated Ras85D. Chemoconnectome Stocks useful for studying neurotransmission. Dominant steriles and lethals Stocks for maintaining dominant sterile and dominant lethal mutations. See Dominant Female Sterile technique for "ovo[D]" stocks. Dominant temperature sensitive lethals Selected stocks with dominant temperature-sensitive lethals, including balancers. Fourth chromosome project Stocks for genes on the 4th chromosome. Gynandromorphs Stocks for generating gynandromorphs. Histone mutations Lines carrying three tandem copies of the histone repeat (His1, His2A, His2B, His3 and His4) with modifications made to particular histone genes. mir gene mutations mir gene knockouts, most are from Steve Cohen and colleagues. P repression stocks Stocks for studying repression of P element transposition. Photoactivatable_AC_GC Stocks carrying constructs to convert adenosine triphosphate to cyclic adenosine monophosphate upon exposure to blue light. Position-effect variegation A panel of P{hsp26-pt-T} insertions illustrating position-effect variegation patterns of miniwhite. RNA isolation Stocks for isolating RNA from specific cells. RNA localization Stocks for localizing RNA intracellularly. Science fair projects Potential stocks for science fair projects. Segregation Distorter Lines with components of the SD meiotic drive system. Sex-biased broods Stocks for recovering mostly male or female progeny using the SD system. Sexing embryos Distinguishing female from male embryos with GFP. Staging third-instar larvae Using Sgs3-GFP to stage larvae to fairly narrow windows. Sxl, tra and snf Notes from Tom Cline on the use of Sxl, tra and snf stocks generated in his lab. Underdominance Stocks for studying underdominant genetic transformation of insect populations. Virus Detection Stocks for detection of viral infection. Wolbachia-carrying All stocks at Bloomington carrying a defined strain of Wolbachia. Still looking for stocks? Lists of miscellaneous categories.
