Labeling and manipulation of subpopulations of neurons to identify their efferent and afferent connectomes are crucial to understand brain function. Synaptic connectivity is the fundamental property of neural circuits. Transgenes have been generated to label or manipulate gene (such as GFP) expression across synapses.
| Transgene | Comment | Stock # | Genotype |
|---|---|---|---|
| P{10xUAS-retro-Tango(ligand)-P2A-EGFP-F}attP2 | Expresses human glucagon ligand (GCG) fused to mouse ICAM5 followed by a self cleaving P2A site followed by farnesylated EGFP under the control of UAS. GCG is targeted to post-synaptic membranes by ICAM5. | 99661 | y[1] w[*] P{y[+t7.7] w[+mC]=QUAS-mtdTomato-3xHA.S}su(Hw)attP8; P{y[+t7.7] w[+mC]=retro-Tango(panneuronal)}attP40/SM6b; P{y[+t7.7] w[+mC]=10xUAS-retro-Tango(ligand)-P2A-EGFP-F}attP2 |
| P{retro-Tango(panneuronal)}attP40 | Expresses the human glucagon receptor (GCGR) tethered to QF via a TEV protease cleavage site under the control of nSyb and human beta-arrestin2 fused to TEV protease under the control of elav. | 99661 | y[1] w[*] P{y[+t7.7] w[+mC]=QUAS-mtdTomato-3xHA.S}su(Hw)attP8; P{y[+t7.7] w[+mC]=retro-Tango(panneuronal)}attP40/SM6b; P{y[+t7.7] w[+mC]=10xUAS-retro-Tango(ligand)-P2A-EGFP-F}attP2 |
| P{trans-Tango}attP40 | Expresses trans-Tango components: nSyb promoter-driven hGCGR::TEVcs::QF, Elav promoter-driven hArr::TEV, and UAS-driven presynaptically tethered Glucagon ligand. | 77123 | w[*]; P{y[+t7.7] w[+mC]=trans-Tango}attP40 |
| P{trans-Tango}attP40 | Expresses trans-Tango components: nSyb promoter-driven hGCGR::TEVcs::QF, Elav promoter-driven hArr::TEV, and UAS-driven presynaptically tethered Glucagon ligand. | 77124 | y[1] w[*] P{y[+t7.7] w[+mC]=UAS-myrGFP.QUAS-mtdTomato-3xHA}su(Hw)attP8; P{y[+t7.7] w[+mC]=trans-Tango}attP40 |
| P{trans-Tango}attP40 | Expresses trans-Tango components: nSyb promoter-driven hGCGR::TEVcs::QF, Elav promoter-driven hArr::TEV, and UAS-driven presynaptically tethered Glucagon ligand. | 77480 | y[1] w[*] P{y[+t7.7] w[+mC]=UAS-myrGFP.QUAS-mtdTomato-3xHA}su(Hw)attP8, P{ry[+t7.2]=hsFLP}1; P{y[+t7.7] w[+mC]=trans-Tango}attP40, P{w[+mW.hs]=FRT(w[hs])}G13, P{w[+mC]=tub-QS.P}5A/CyO |
| P{trans-Tango}attP40 | Expresses trans-Tango components: nSyb promoter-driven hGCGR::TEVcs::QF, Elav promoter-driven hArr::TEV, and UAS-driven presynaptically tethered Glucagon ligand. | 77481 | y[1] w[*] P{y[+t7.7] w[+mC]=UAS-myrGFP.QUAS-mtdTomato-3xHA}su(Hw)attP8; P{y[+t7.7] w[+mC]=trans-Tango}attP40, PBac{y[+mDint2] w[+mC]=tubP(FRT.QS)}VK00018 |
| P{trans-Tango}attP40 | Expresses trans-Tango components: nSyb promoter-driven hGCGR::TEVcs::QF, Elav promoter-driven hArr::TEV, and UAS-driven presynaptically tethered Glucagon ligand. | 77482 | w[*]; P{y[+t7.7] w[+mC]=trans-Tango}attP40/CyO; P{y[+t7.7] w[+mC]=10XUAS-IVS-myr::tdTomato}attP2, P{w[+mC]=QUAS(FRT.stop)mCD8-GFP.P}5/TM6B |
| P{trans-Tango}attP40 | Expresses trans-Tango components: nSyb promoter-driven hGCGR::TEVcs::QF, Elav promoter-driven hArr::TEV, and UAS-driven presynaptically tethered Glucagon ligand. | 90924 | w[*]; P{y[+t7.7] w[+mC]=trans-Tango}attP40 P{w[+mC]=QUAS-mtdTomato-3xHA}14/CyO; P{w[+mC]=iav-GAL4.K}3 |
| P{trans-Tango}attP40 | Expresses trans-Tango components: nSyb promoter-driven hGCGR::TEVcs::QF, Elav promoter-driven hArr::TEV, and UAS-driven presynaptically tethered Glucagon ligand. | 95315 | y[1] w[*] P{y[+t7.7] w[+mC]=5xQUAS-nlsDsRedT4}su(Hw)attP8; P{y[+t7.7] w[+mC]=trans-Tango}attP40/SM6b |
| P{trans-Tango}attP40 | Expresses trans-Tango components: nSyb promoter-driven hGCGR::TEVcs::QF, Elav promoter-driven hArr::TEV, and UAS-driven presynaptically tethered Glucagon ligand. | 95316 | y[1] w[*] P{y[+t7.7] w[+mC]=10xQUAS-GCaMP6s}su(Hw)attP8; P{y[+t7.7] w[+mC]=trans-Tango}attP40 |
| P{trans-TangoMkII}attP40 | Expresses trans-TangoMKII components: nSyb-driven hGCGR::TEVcs::QF, Elav-driven hArr::TEV, and UAS-driven presynaptically tethered Glucagon ligand. This version has higher signal over noise ratio than the original trans-Tango. | 95317 | P{ry[+t7.2]=hsFLP}1, y[1] w[1118] P{w[+mC]=UAS-mCD8::GFP.L}Ptp4E[LL4] P{w[+mC]=QUAS-mtdTomato-3xHA}22; P{y[+t7.7] w[+mC]=trans-TangoMkII}attP40/SM6b |
| P{UAS-Dop1R1-Tango}attP2 | Expresses a fusion protein DopR1-TEVcs-lexA-2A-Arrestin1-TEVp under the control of UAS. Upon dopamine binding, lexA is cleaved from DopR1 and can activate transcription from a lexAop site. | 68234 | y[1] v[1]; P{y[+t7.7] v[+t1.8]=UAS-Dop1R1-Tango}attP2/TM6C, Sb[1] Tb[1] |
| P{UAS-Oamb-Tango}attP2 | Expresses a fusion protein Oamb-TEVcs-lexA-2A-Arrestin1-TEVp under the control of UAS. Upon octopamine binding, lexA is cleaved from Oamb and can activate transcription from a lexAop site. | 68235 | y[1] v[1]; P{y[+t7.7] v[+t1.8]=UAS-Oamb-Tango}attP2/TM3, Ser[1] |
| PBac{UAS(B3RT.B2)BoNTA}VK00005 | Expresses BoNT-A, antiGFP nanobody and CD2 fusion protein under the control of UAS upon removal of B3RT.stop cassette. Designed to use in BAcTrace system for retrograde, trans-synaptic labeling of presynaptic neurons. | 90825 | w[*]; PBac{LexAop2-Syb.GFP.P10}VK00037 PBac{LexAop2-QF2.V5.hSNAP25.HIVNES.Syx1A}VK00018/CyO; P{20XUAS-B3R.PEST}attP2 PBac{UAS(B3RT.B2)BoNTA}VK00005 P{QUAS-mtdTomato-3xHA}26/TM2 |
| PBac{UAS(B3RT.B2)BoNTA}VK00005 | Expresses BoNT-A, antiGFP nanobody and CD2 fusion protein under the control of UAS upon removal of B3RT.stop cassette. Designed to use in BAcTrace system for retrograde, trans-synaptic labeling of presynaptic neurons. | 90826 | w[*]; PBac{LexAop2-Syb.GFP.P10}VK00037 PBac{LexAop2-Syb.GFP[N146I].TEV[T173V]}VK00002 PBac{LexAop2-QF2.V5.hSNAP25.HIVNES.Syx1A}VK00018/CyO; P{20XUAS-B3R.PEST}attP2 PBac{UAS(B3RT.B2)BoNTA}VK00005 P{QUAS-mtdTomato-3xHA}26/TM6B, Tb[1] |
| PBac{UAS(B3RT.B2)BoNTA}VK00027 | Expresses BoNT-A, antiGFP nanobody and CD2 fusion protein under the control of UAS upon removal of B3RT.stop cassette. Designed to use in BAcTrace system for retrograde, trans-synaptic labeling of presynaptic neurons. | 90825 | w[*]; PBac{LexAop2-Syb.GFP.P10}VK00037 PBac{LexAop2-QF2.V5.hSNAP25.HIVNES.Syx1A}VK00018/CyO; P{20XUAS-B3R.PEST}attP2 PBac{UAS(B3RT.B2)BoNTA}VK00005 P{QUAS-mtdTomato-3xHA}26/TM2 |
| PBac{UAS(B3RT.B2)BoNTA}VK00027 | Expresses BoNT-A, antiGFP nanobody and CD2 fusion protein under the control of UAS upon removal of B3RT.stop cassette. Designed to use in BAcTrace system for retrograde, trans-synaptic labeling of presynaptic neurons. | 90826 | w[*]; PBac{LexAop2-Syb.GFP.P10}VK00037 PBac{LexAop2-Syb.GFP[N146I].TEV[T173V]}VK00002 PBac{LexAop2-QF2.V5.hSNAP25.HIVNES.Syx1A}VK00018/CyO; P{20XUAS-B3R.PEST}attP2 PBac{UAS(B3RT.B2)BoNTA}VK00005 P{QUAS-mtdTomato-3xHA}26/TM6B, Tb[1] |