Stocks for Demonstrating Selection and Hitchhiking

Gredler et al. have designed an educational exercise for observing selection for increased frequency of an allele ("red eye color" in the figure below) in a population and demonstrating that linked molecular markers also increase in frequency based on their chromosomal distance from the eye color gene.

Figure from Gredler et al. Revised college genetics laboratory exercise for witnessing phenotypic and molecular evolution in the fruit fly, an online educational resource of the Genetics Society of America

The experiment was described in the paper "Revised college genetics laboratory exercise for witnessing phenotypic and molecular evolution in the fruit fly". The strains for the exercise are shown below.

IMPORTANT: We lost one of the stocks for this lab in 2021 (60740 w[1118] Mi{GFP[E.3xP3]=ET1}MB05922/Dp(2;Y)G, P{hs-hid}Y) and replaced it with a stock carrying the same insertion but lacking the hs-hid on Y (25312 w[1118] Mi{GFP[E.3xP3]=ET1}MB05922). Note you must therefore collect 25312 virgins "manually" . . .


Teachers should note that the same strains are available from Carolina Biological Supply as part of a preassembled kit. The kit provides supplies, media, literature and relatively large fly cultures and Carolina specializes in supporting educators. We send only small starter cultures with no additional materials. Unless you are already proficient at rearing Drosophila and have the necessary supplies on hand, we recommend that you order from Carolina.


Related links:   Early Development Lab   Isozymes Lab