GAL80 lines

GAL4 protein activates transcription through binding to upstream activation sequence (UAS), whereas GAL80 protein prevents GAL4-dependent transcription. The temperature-sensitive GAL80[ts] provides a temporal regulation of UAS transgene. For more information on using GAL4/UAS/GAL80, see Duffy (2002): GAL4 System in Drosophila: A Fly Geneticist’s Swiss Army Knife. Genesis 34:1–15.

Stk #	Promoter	Symbol	Chr	Genotype
30040		P{ET-GAL80}1	1	P{y[+t7.7]=ET-GAL80}1, y[1] w[1118]
30041		P{ET-GAL80}2	2	y[1] w[1118]; P{y[+t7.7]=ET-GAL80}2
56501		P{ET-GAL80}Gaz	2	w[*]; P{y[+t7.7]=ET-GAL80}Gaz, P{w[+mC]=ChAT-GAL4.7.4}19B P{w[+mC]=UAS-GFP.S65T}Myo31DF[T2]/CyO
56505		P{ET-GAL80}Gaz	2	y[1] w[*]; P{y[+t7.7]=ET-GAL80}Gaz/CyO
64068		PBac{IS.IVS-GAL80-WPRE.w-}pum[0441-G80-WPRE]	3	w[*]; PBac{IS.IVS-GAL80-WPRE.w-}pum[0441-G80-WPRE]
67092	Actin (unknown)	P{Act(loxP.GAL80.stop)lexA::p65}attP40	2	y[1] w[*]; P{y[+t7.7] w[+mC]=Act(loxP.GAL80.stop)lexA::p65}attP40/CyO; TM3, Sb[1]/TM6B, Tb[1]
67094	Actin (unknown)	P{Act(loxP.GAL80.stop)lexA::p65}attP40	2	y[1] w[*] P{y[+t7.7] w[+mC]=dpn(KDRT.stop)cre.PEST}su(Hw)attP8; P{y[+t7.7] w[+mC]=Act(loxP.GAL80.stop)lexA::p65}attP40 P{y[+t7.7] w[+mC]=lexAop-rCD2::RFP-p10.UAS-mCD8::GFP-p10}su(Hw)attP5; TM3, Sb[1]/TM6B, Tb[+]
67095	Actin (unknown)	P{Act(loxP.GAL80.stop)lexA::p65}attP40	2	y[1] w[*] P{y[+t7.7] w[+mC]=hs(KDRT.stop)FLP}attP18, P{y[+t7.7] w[+mC]=dpn(FRT.stop)cre.PEST}su(Hw)attP8; P{y[+t7.7] w[+mC]=Act(loxP.GAL80.stop)lexA::p65}attP40 P{y[+t7.7] w[+mC]=lexAop-rCD2::RFP-p10.UAS-mCD8::GFP-p10}su(Hw)attP5; TM3, Sb[1]/TM6B, Tb[1]
5132	alphaTub84B	P{tubP-GAL80}LL1	1	P{ry[+t7.2]=hsFLP}1, P{w[+mC]=tubP-GAL80}LL1 w[*] P{ry[+t7.2]=neoFRT}19A
5133	alphaTub84B	P{tubP-GAL80}LL1	1	P{ry[+t7.2]=hsFLP}1, P{w[+mC]=tubP-GAL80}LL1 w[*] P{ry[+t7.2]=neoFRT}19A; betaTub60D[Pin-Yt]/CyO
5134	alphaTub84B	P{tubP-GAL80}LL1	1	P{ry[+t7.2]=hsFLP}1, P{w[+mC]=tubP-GAL80}LL1 w[*] P{ry[+t7.2]=neoFRT}19A; P{w[+mC]=UAS-mCD8::GFP.L}LL5
42717	alphaTub84B	P{tubP-GAL80}LL1	1	P{w[+mC]=tubP-GAL80}LL1 w[*] P{ry[+t7.2]=ey-FLP.N}2 P{ry[+t7.2]=neoFRT}19A
42721	alphaTub84B	P{tubP-GAL80}LL1	1	P{ry[+t7.2]=hsFLP}1, P{w[+mC]=tubP-GAL80}LL1 w[*] P{ry[+t7.2]=neoFRT}19A; P{w[+mW.hs]=GawB}sca[DB], P{w[+mC]=UAS-pon.GFP.LD}2, P{w[+mC]=UAS-Tau-GFP.m6}2
42726	alphaTub84B	P{tubP-GAL80}LL1	1	P{w[+mC]=tubP-GAL80}LL1 w[*] P{ry[+t7.2]=neoFRT}19A; P{w[+mC]=Act5C-GAL4}25FO1, P{w[+mC]=UAS-GFP.U}2/CyO
42731	alphaTub84B	P{tubP-GAL80}LL1	1	P{w[+mC]=Ubx-FLP}1, P{w[+mC]=tubP-GAL80}LL1 w[*] P{ry[+t7.2]=neoFRT}19A
44407	alphaTub84B	P{tubP-GAL80}LL1	1	P{ry[+t7.2]=hsFLP}1, P{w[+mC]=tubP-GAL80}LL1 w[*] P{ry[+t7.2]=neoFRT}19A; P{w[+mC]=UAS-mCD8::GFP.L}LL5/CyO; P{w[+mW.hs]=GawB}OK107 ey[OK107]
58459	alphaTub84B	P{tubP-GAL80}LL1	1	P{w[+mC]=Ubx-FLP}1, y[1] P{w[+mC]=tubP-GAL80}LL1 w[*] P{ry[+t7.2]=neoFRT}19A; P{w[+mC]=UAS-EGFP-sca.C}2/CyO
86305	alphaTub84B	P{tubP-GAL80}LL1	1	P{ry[+t7.2]=hsFLP}12, P{w[+mC]=tubP-GAL80}LL1 w[*] P{ry[+t7.2]=neoFRT}19A; P{w[+mC]=UAS-GFP.U}2; P{w[+mC]=tubP-GAL4}LL7 P{ry[+t7.2]=neoFRT}82B P{w[+mC]=tubP-GAL80}LL3/TM6C, Sb[1] Tb[1]
86306	alphaTub84B	P{tubP-GAL80}LL1	1	P{ry[+t7.2]=hsFLP}12, P{w[+mC]=tubP-GAL80}LL1 w[*] P{ry[+t7.2]=neoFRT}19A; P{w[+mC]=UAS-mCD8.ChRFP}2/CyO; P{w[+mC]=tubP-GAL4}LL7 P{ry[+t7.2]=neoFRT}82B P{w[+mC]=tubP-GAL80}LL3/TM6C, Sb[1] Tb[1]
86307	alphaTub84B	P{tubP-GAL80}LL1	1	P{ry[+t7.2]=hsFLP}12, P{w[+mC]=tubP-GAL80}LL1 w[*] P{ry[+t7.2]=neoFRT}19A; P{w[+mC]=UAS-N.RNAi.P}2; P{w[+mC]=tubP-GAL4}LL7 P{ry[+t7.2]=neoFRT}82B P{w[+mC]=tubP-GAL80}LL3/TM6C, Sb[1] Tb[1]
90893	alphaTub84B	P{tubP-GAL80}LL1	1	P{ry[+t7.2]=hsFLP}1, P{w[+mC]=tubP-GAL80}LL1 w[*] P{ry[+t7.2]=neoFRT}19A; P{y[+t7.7] w[+mC]=hs-FLPD5}attP40 PBac{y[+mDint2] w[+mC]=20XUAS-6XGFP}VK00018, P{w[+mC]=UAS-RedStinger}4/CyO; P{w[+mC]=iav-GAL4.K}3
90894	alphaTub84B	P{tubP-GAL80}LL1	1	P{ry[+t7.2]=hsFLP}1, P{w[+mC]=tubP-GAL80}LL1 w[*] P{ry[+t7.2]=neoFRT}19A; P{y[+t7.7] w[+mC]=hs-FLPD5}attP40 PBac{y[+mDint2] w[+mC]=20XUAS-6XGFP}VK00018/CyO; P{w[+mC]=iav-GAL4.K}3
90895	alphaTub84B	P{tubP-GAL80}LL1	1	P{ry[+t7.2]=hsFLP}1, P{w[+mC]=tubP-GAL80}LL1 w[*] P{ry[+t7.2]=neoFRT}19A; Kr[If-1]/CyO; P{w[+mC]=iav-GAL4.K}3
90901	alphaTub84B	P{tubP-GAL80}LL1	1	P{ry[+t7.2]=hsFLP}1, P{w[+mC]=tubP-GAL80}LL1 w[*] P{ry[+t7.2]=neoFRT}19A; P{y[+t7.7] w[+mC]=nSyb-GAL4.P}attP2
90902	alphaTub84B	P{tubP-GAL80}LL1	1	P{ry[+t7.2]=hsFLP}1, P{w[+mC]=tubP-GAL80}LL1 w[*] P{ry[+t7.2]=neoFRT}19A; P{y[+t7.7] w[+mC]=hs-FLPD5}attP40 PBac{20XUAS-6XGFP}VK00018/CyO; P{y[+t7.7] w[+mC]=R82G02-QF2.L}attP2 P{QUAS-mtdTomato-3xHA}26
99587	alphaTub84B	P{tubP-GAL80}LL1	1	P{ry[+t7.2]=hsFLP}12, P{w[+mC]=tubP-GAL80}LL1 w[*] P{ry[+t7.2]=neoFRT}19A; P{w[+mC]=UAS-lacZ.NZ}20b, P{w[+mC]=UAS-mCD8::GFP.L}LL5; P{w[+mC]=tubP-GAL4}LL7/TM6B, Tb[+]
600563	alphaTub84B	P{tubP-GAL80}LL1	1	P{ry[+t7.2]=hsFLP}1, P{w[+mC]=tubP-GAL80}LL1 w[*] P{ry[+t7.2]=neoFRT}19A; P{w[+mC]=UAS-mCD8::GFP.L}LL6, P{w[+mC]=tubP-GAL4}LL7/TM3, P{w[+mC]=ActGFP}JMR2, Ser[1]
605375	alphaTub84B	P{tubP-GAL80}LL1	1	P{w[+mC]=Ubx-FLP}1, y[1] P{w[+mC]=tubP-GAL80}LL1 w[*] P{ry[+t7.2]=neoFRT}19A; PBac{y[+mDint2] w[+mC]=UAS-FLAG-hPTAR1}VK00037
605393	alphaTub84B	P{tubP-GAL80}LL1	1	P{w[+mC]=tubP-GAL80}LL1 w[*] P{ry[+t7.2]=ey-FLP.N}2 P{ry[+t7.2]=neoFRT}19A; P{w[+mC]=Act5C-GAL4}25FO1 P{w[+mC]=UAS-mCD8::GFP.L}LL5/CyO
600209	alphaTub84B	P{tubP-GAL80.1xDHFR22-T2A-mCh}JB37B	2	y[1] w[*]; P{tubP-GAL80.1xDHFR22-T2A-mCh}JB37B/CyO
5192	alphaTub84B	P{tubP-GAL80}LL10	2	y[1] w[*]; P{w[+mC]=tubP-GAL80}LL10 P{ry[+t7.2]=neoFRT}40A/CyO
44406	alphaTub84B	P{tubP-GAL80}LL10	2	P{ry[+t7.2]=hsFLP}1, y[1] w[*] P{w[+mC]=UAS-mCD8::GFP.L}Ptp4E[LL4]; P{w[+mC]=tubP-GAL80}LL10 P{ry[+t7.2]=neoFRT}40A; P{w[+mW.hs]=GawB}OK107 ey[OK107]
86316	alphaTub84B	P{tubP-GAL80}LL10	2	P{ry[+t7.2]=hsFLP}12, P{w[+mC]=UAS-GFP.U}1, y[1] w[*]; P{w[+mC]=tubP-GAL80}LL10 P{ry[+t7.2]=neoFRT}40A; P{w[+mC]=tubP-GAL4}LL7 P{ry[+t7.2]=neoFRT}82B P{w[+mC]=tubP-GAL80}LL3/TM6C, Sb[1] Tb[1]
99588	alphaTub84B	P{tubP-GAL80}LL10	2	P{ry[+t7.2]=hsFLP}1, y[1] w[*] P{w[+mC]=UAS-mCD8::GFP.L}Ptp4E[LL4]; P{w[+mC]=tubP-GAL80}LL10 P{ry[+t7.2]=neoFRT}40A; P{w[+mC]=tubP-GAL4}LL7
600252	alphaTub84B	P{tubP-GAL80}LL10	2	P{ry[+t7.2]=hsFLP}12, y[1] w[*]; P{w[+mC]=tubP-GAL80}LL10 P{ry[+t7.2]=neoFRT}40A; M{RFP[3xP3.PB] w[+mC]=UASp-Lifeact.TagRFP-T}ZH-68E P{w[+mC]=tubP-GAL4}LL7/TM6B, Tb[+]
605350	alphaTub84B	P{tubP-GAL80}LL10	2	P{w[+mC]=UAS-mCD8::GFP.L}Ptp4E[LL4], P{ry[+t7.2]=hsFLP}1, w[*]; P{w[+mC]=tubP-GAL80}LL10 P{ry[+t7.2]=neoFRT}40A; P{w[+mC]=tubP-GAL4}LL7
5140	alphaTub84B	P{tubP-GAL80}LL2	2	w[*]; P{w[+mW.hs]=FRT(w[hs])}G13 P{w[+mC]=tubP-GAL80}LL2
5142	alphaTub84B	P{tubP-GAL80}LL2	2	w[*]; P{w[+mW.hs]=FRT(w[hs])}G13 P{w[+mC]=piM}45F P{w[+mC]=tubP-GAL80}LL2/CyO
5143	alphaTub84B	P{tubP-GAL80}LL2	2	w[*]; P{w[+mW.hs]=FRT(w[hs])}G13 P{w[+mC]=piM}45F P{w[+mC]=tubP-GAL80}LL2; P{w[+mC]=tubP-GAL4}LL7/TM6C, Sb[1] Tb[1]
5145	alphaTub84B	P{tubP-GAL80}LL2	2	P{w[+mW.hs]=GawB}elav[C155], P{ry[+t7.2]=hsFLP}1; P{w[+mW.hs]=FRT(w[hs])}G13 P{w[+mC]=tubP-GAL80}LL2/CyO
5147	alphaTub84B	P{tubP-GAL80}LL2	2	P{w[+mW.hs]=GawB}elav[C155]; P{w[+mW.hs]=FRT(w[hs])}G13 P{w[+mC]=tubP-GAL80}LL2
9917	alphaTub84B	P{tubP-GAL80}LL2	2	y[1] w[*]; P{ry[+t7.2]=neoFRT}42D P{w[+mC]=tubP-GAL80}LL2/CyO, y[+]
64286	alphaTub84B	P{tubP-GAL80}LL2	2	y[1] w[67c23]; Hr39[C105] P{w[+mW.hs]=FRT(w[hs])}G13 P{w[+mC]=tubP-GAL80}LL2
86318	alphaTub84B	P{tubP-GAL80}LL2	2	P{ry[+t7.2]=hsFLP}12, P{w[+mC]=UAS-GFP.U}1, w[*]; P{ry[+t7.2]=neoFRT}42D P{w[+mC]=tubP-GAL80}LL2/CyO; P{w[+mC]=tubP-GAL4}LL7 P{ry[+t7.2]=neoFRT}82B P{w[+mC]=tubP-GAL80}LL3/TM6C, Sb[1] Tb[+]
93000	alphaTub84B	P{tubP-GAL80}LL2	2	y[1] w[*]; P{ry[+t7.2]=neoFRT}42D Cirl[KO.attP.w+], P{w[+mC]=tubP-GAL80}LL2/CyO
93002	alphaTub84B	P{tubP-GAL80}LL2	2	w[*]; P{ry[+t7.2]=neoFRT}42D TI{TI}Cirl[N-RFP], P{w[+mC]=tubP-GAL80}LL2/CyO
605351	alphaTub84B	P{tubP-GAL80}LL2	2	P{w[+mC]=UAS-mCD8::GFP.L}Ptp4E[LL4], P{ry[+t7.2]=hsFLP}1, w[*]; P{ry[+t7.2]=neoFRT}42D P{w[+mC]=tubP-GAL80}LL2; P{w[+mC]=tubP-GAL4}LL7
9489	alphaTub84B	P{tubP-GAL80}OV2	2	w[*]; In(2LR)bw[V1], ds[33k] dpy[ov1] Adc[b-1] bw[V1]/CyO, P{w[+mC]=tubP-GAL80}OV2
9491	alphaTub84B	P{tubP-GAL80}OV2	2	w[*]; l(2)DTS91[1] sna[Sco]/CyO, P{w[+mC]=tubP-GAL80}OV2
94622	alphaTub84B	TI{alphaTub84B-SPARC3-D-OUT-GAL80}CR-su(Hw)P5	2	w[*]; TI{RFP[DsRed.3xP3.cUa]=alphaTub84B-SPARC3-D-OUT-GAL80}CR-su(Hw)P5/CyO; TM2/TM6B, Tb[1]
94621	alphaTub84B	TI{alphaTub84B-SPARC3-S-OUT-GAL80}CR-su(Hw)P5	2	w[*]; TI{RFP[DsRed.3xP3.cUa]=alphaTub84B-SPARC3-S-OUT-GAL80}CR-su(Hw)P5/CyO; TM2/TM6B, Tb[1]
600210	alphaTub84B	P{tubP-GAL80.1xDHFR22-T2A-mCh}JB89B	3	w[]; P{y[+t] w[+mC]=UAS-sfxa109(hid.mCh-rev)}VIE-260B; P{tubP-GAL80.1xDHFR22-T2A-mCh}JB89B/TM3, Sb[1]
600211	alphaTub84B	P{tubP-GAL80.1xDHFR22-T2A-mCh}JB89B	3	w[*]; P{y[+t7.7] w[+mC]=UAS-3xFLAG-dCas9::nej(core)}attP40; P{tubP-GAL80.1xDHFR22-T2A-mCh}JB89B/TM3, Sb[1]
600212	alphaTub84B	P{tubP-GAL80.1xDHFR22-T2A-mCh}JB89B	3	w[*]; sna[Sco]/CyO; P{tubP-GAL80.1xDHFR22-T2A-mCh}JB89B/TM3, Sb[1]
63083	alphaTub84B	P{tubP-GAL80}3	3	w[*]; P{w[+mC]=GAL4-btl.S}2, P{w[+mC]=UASp-Act5C.T:GFP}2; P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B bor[1520]/TM3, P{w[+mC]=tubP-GAL80}3, Sb[1]
5135	alphaTub84B	P{tubP-GAL80}LL3	3	y[1] w[*]; P{ry[+t7.2]=neoFRT}82B P{w[+mC]=tubP-GAL80}LL3
30036	alphaTub84B	P{tubP-GAL80}LL3	3	y[1] w[1118]; betaTub60D[Pin-1]/CyO; P{w[+mC]=tubP-GAL4}LL7 P{ry[+t7.2]=neoFRT}82B P{w[+mC]=tubP-GAL80}LL3
42734	alphaTub84B	P{tubP-GAL80}LL3	3	P{w[+mC]=Ubx-FLP}1, P{w[+mC]=tubP-GAL4}1, P{w[+mC]=UAS-GFP.U}1, y[1] w[*]; P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E, P{w[+mC]=tubP-GAL80}LL3/TM6
44408	alphaTub84B	P{tubP-GAL80}LL3	3	P{ry[+t7.2]=hsFLP}1, y[1] w[*] P{w[+mC]=UAS-mCD8::GFP.L}Ptp4E[LL4]; P{ry[+t7.2]=neoFRT}82B P{w[+mC]=tubP-GAL80}LL3/TM3, Sb[1]; P{w[+mW.hs]=GawB}OK107 ey[OK107]
56509	alphaTub84B	P{tubP-GAL80}LL3	3	w[*]; betaTub60D[Pin-1]/CyO; P{KDRT.A}82F7 P{w[+mC]=tubP-GAL80}LL3/TM3, Sb[1]
86305	alphaTub84B	P{tubP-GAL80}LL3	3	P{ry[+t7.2]=hsFLP}12, P{w[+mC]=tubP-GAL80}LL1 w[*] P{ry[+t7.2]=neoFRT}19A; P{w[+mC]=UAS-GFP.U}2; P{w[+mC]=tubP-GAL4}LL7 P{ry[+t7.2]=neoFRT}82B P{w[+mC]=tubP-GAL80}LL3/TM6C, Sb[1] Tb[1]
86306	alphaTub84B	P{tubP-GAL80}LL3	3	P{ry[+t7.2]=hsFLP}12, P{w[+mC]=tubP-GAL80}LL1 w[*] P{ry[+t7.2]=neoFRT}19A; P{w[+mC]=UAS-mCD8.ChRFP}2/CyO; P{w[+mC]=tubP-GAL4}LL7 P{ry[+t7.2]=neoFRT}82B P{w[+mC]=tubP-GAL80}LL3/TM6C, Sb[1] Tb[1]
86307	alphaTub84B	P{tubP-GAL80}LL3	3	P{ry[+t7.2]=hsFLP}12, P{w[+mC]=tubP-GAL80}LL1 w[*] P{ry[+t7.2]=neoFRT}19A; P{w[+mC]=UAS-N.RNAi.P}2; P{w[+mC]=tubP-GAL4}LL7 P{ry[+t7.2]=neoFRT}82B P{w[+mC]=tubP-GAL80}LL3/TM6C, Sb[1] Tb[1]
86311	alphaTub84B	P{tubP-GAL80}LL3	3	P{ry[+t7.2]=hsFLP}12, P{w[+mC]=UAS-GFP.U}1, y[1] w[*]; P{w[+mC]=tubP-GAL4}LL7 P{ry[+t7.2]=neoFRT}82B P{w[+mC]=tubP-GAL80}LL3/TM6C, Sb[1] Tb[1]
86312	alphaTub84B	P{tubP-GAL80}LL3	3	P{ry[+t7.2]=hsFLP}12, P{w[+mC]=UAS-GFP.U}1, y[1] w[*]; P{w[+mC]=UAS-N.RNAi.P}2/CyO; P{w[+mC]=tubP-GAL4}LL7 P{ry[+t7.2]=neoFRT}82B P{w[+mC]=tubP-GAL80}LL3/TM6C, Sb[1] Tb[1]
86316	alphaTub84B	P{tubP-GAL80}LL3	3	P{ry[+t7.2]=hsFLP}12, P{w[+mC]=UAS-GFP.U}1, y[1] w[*]; P{w[+mC]=tubP-GAL80}LL10 P{ry[+t7.2]=neoFRT}40A; P{w[+mC]=tubP-GAL4}LL7 P{ry[+t7.2]=neoFRT}82B P{w[+mC]=tubP-GAL80}LL3/TM6C, Sb[1] Tb[1]
86318	alphaTub84B	P{tubP-GAL80}LL3	3	P{ry[+t7.2]=hsFLP}12, P{w[+mC]=UAS-GFP.U}1, w[*]; P{ry[+t7.2]=neoFRT}42D P{w[+mC]=tubP-GAL80}LL2/CyO; P{w[+mC]=tubP-GAL4}LL7 P{ry[+t7.2]=neoFRT}82B P{w[+mC]=tubP-GAL80}LL3/TM6C, Sb[1] Tb[+]
92783	alphaTub84B	P{tubP-GAL80}LL3	3	y[1] w[1118]; betaTub60D[Pin-1]/CyO; P{w[+mC]=tubP-GAL4}LL7 P{ry[+t7.2]=neoFRT}82B P{w[+mC]=tubP-GAL80}LL3/TM6B, Tb[1]
5191	alphaTub84B	P{tubP-GAL80}LL9	3	y[1] w[*]; P{w[+mC]=tubP-GAL80}LL9 P{ry[+t7.2]=neoFRT}80B
5190	alphaTub84B	P{tubP-GAL80}LL9	3	y[1] w[*]; P{w[+mC]=tubP-GAL80}LL9 P{w[+mW.hs]=FRT(w[hs])}2A/TM3, Sb[1]
42732	alphaTub84B	P{tubP-GAL80}LL9	3	P{ry[+t7.2]=hsFLP}1, P{w[+mC]=tubP-GAL4}1, P{w[+mC]=UAS-GFP.T:Myc.T:nls2}1, y[1] w[]; RpS17[4] P{w[+m] ry[+t7.2]=white-un1}70C P{w[+mC]=tubP-GAL80}LL9 P{ry[+t7.2]=neoFRT}80B/TM6B, Tb[1]
44404	alphaTub84B	P{tubP-GAL80}LL9	3	P{ry[+t7.2]=hsFLP}1, y[1] w[*] P{w[+mC]=UAS-mCD8::GFP.L}Ptp4E[LL4]; P{w[+mC]=tubP-GAL80}LL9 P{w[+mW.hs]=FRT(w[hs])}2A/TM3, Sb[1]; P{w[+mW.hs]=GawB}OK107 ey[OK107]
81889	alphaTub84B	P{tubP-GAL80}LL9	3	w[*]; P{Act5C-GAL4-w}E1, P{w[+mC]=UAS-EGFP}5a.2/CyO, P{Wee-P.ph0}2; P{w[+mC]=tubP-GAL80}LL9 PBac{y[+mDint2] w[+mC]=U6-GAL80.sgRNA}VK00027/TM6B, Tb[1]
600251	alphaTub84B	P{tubP-GAL80}LL9	3	P{ry[+t7.2]=hsFLP}12, y[1] w[*]/Dp(1;Y)y[+]; P{w[+mC]=tubP-GAL4}2, M{RFP[3xP3.PB] w[+mC]=UASp-Lifeact.TagRFP-T}ZH-22A/CyO; P{w[+mC]=tubP-GAL80}LL9 P{w[+mW.hs]=FRT(w[hs])}2A
9490	alphaTub84B	P{tubP-GAL80}OV3	3	w[*]; TM6B, P{w[+mC]=tubP-GAL80}OV3, Tb[1]/TM3, Sb[1]
97119	alphaTub84B	P{tubP-GAL80}OV3	3	w[*]; P{w[+mC]=UAS-Rap1.N17}2/CyO; TM2/TM6B, P{w[+mC]=tubP-GAL80}OV3, Tb[1]
97120	alphaTub84B	P{tubP-GAL80}OV3	3	w[*]; P{w[+mC]=GAL4-slbo.2.6}16/CyO; P{w[+mC]=UAS-Rap1.V12}3/TM6B, P{w[+mC]=tubP-GAL80}OV3, Tb[1]
600564	alphaTub84B	P{tubP-GAL80}OV3	3	w[*]; Bl[1]/CyO, P{w[BmC]=Dfd-EYFP.w[BmC]}2; P{w[+mC]=tubP-GAL4}LL7, P{w[+mC]=UAS-RFP.W}3/TM6B, P{w[+mC]=tubP-GAL80}OV3, Tb[1]
92470	alphaTub84B	PBac{tubP-TIR1-2A-GAL80.AID}VK00040	3	w[1118]; PBac{y[+mDint2] w[+mC]=tubP-TIR1-2A-GAL80.AID}VK00040
606068	alphaTub84B		4	w[1118]; TI{TI}FRT101F TI{RFP[DsRed.3xP3.cUa]=Tub-GAL80.W}102B-DsRed+/TI{GMR-HMS04515}Gat[eya]
606069	alphaTub84B		4	w[1118]; TI{TI}FRT101F TI{RFP[DsRed.3xP3.cUa]=Tub-GAL80.W}102C-DsRed+/TI{GMR-HMS04515}Gat[eya]
605748	AstC-R1	P{R60G08.Eb5-GAL80}attP40	2	w[1118]; P{y[+t7.7] w[+mC]=R60G08.Eb5-GAL80}attP40/CyO
98433	ChAT	Mi{Trojan-3XGAL80.0}ChAT[MI04508-T3XG80.0]	3	y[1] w[*]; Mi{Trojan-3XGAL80.0}ChAT[MI04508-T3XG80.0] CG7715[MI04508-T3XG80.0-X]/TM3, Sb[1]
605546	ChAT	P{ChAT.3.3kb-Gal80}3	3	w[*]; wg[Sp-1]/CyO; P{w[+mC]=ChAT.3.3kb-Gal80}3/TM3, Ser[1]
95093	DAT	P{DAT-B-GAL80}JK22C	2	w[*]; P{y[+t7.7] w[+mC]=DAT-B-GAL80}JK22C
95097	DAT	P{R55C10-GAL80}JK22C	2	w[*]; P{y[+t7.7] w[+mC]=R55C10-GAL80}JK22C/CyO
605544	DAT	P{R58E02-GAL80}attP2	3	w[*]; P{y[+t7.7] w[+mC]=R58E02-GAL80}attP2
95098	Ddc	P{R60F07-GAL80}JK22C	2	w[*]; P{y[+t7.7] w[+mC]=R60F07-GAL80}JK22C/CyO
95099	Ddc	P{R61H03-GAL80}JK22C	2	w[*]; P{y[+t7.7] w[+mC]=R61H03-GAL80}JK22C/CyO
602882	elav	P{elav-GAL80.S}3	3	w[]; P{w[+m]=elav-GAL80.S}3
35822	ey	P{ey-GAL80.3.5}1	1	P{w[+mC]=ey-GAL80.3.5}1, y[1] w[*]; CyO/In(2LR)Gla, wg[Gla-1] PPO1[Bc]
605545	GABA-B-R3	PBac{R76B09-GAL80}VK00027	3	w[*]; PBac{y[+mDint2] w[+mC]=R76B09-GAL80}VK00027
80563	Gr21a	P{Gr21a-GAL80.1756}attP2	3	w[*]; P{y[+t7.7] w[+mC]=Gr21a-GAL80.1756}attP2
66582	Ir25a	P{Ir25a-GAL80.1.1}attP40	2	w[1118]; P{y[+t7.7] w[+mC]=Ir25a-GAL80.1.1}attP40/CyO; TM2/TM6B, Tb[1]
32217	lexA operator	P{8XLexAop2-IVS-GAL80-WPRE}su(Hw)attP8	1	y[1] w[*] P{y[+t7.7] w[+mC]=8XLexAop2-IVS-GAL80-WPRE}su(Hw)attP8
32214	lexA operator	P{8XLexAop2-IVS-GAL80-WPRE}attP40	2	w[*]; P{y[+t7.7] w[+mC]=8XLexAop2-IVS-GAL80-WPRE}attP40
32216	lexA operator	P{8XLexAop2-IVS-GAL80-WPRE}su(Hw)attP5	2	w[1118]; P{y[+t7.7] w[+mC]=8XLexAop2-IVS-GAL80-WPRE}su(Hw)attP5
32213	lexA operator	P{8XLexAop2-IVS-GAL80-WPRE}attP2	3	w[*]; P{y[+t7.7] w[+mC]=8XLexAop2-IVS-GAL80-WPRE}attP2
32215	lexA operator	P{8XLexAop2-IVS-GAL80-WPRE}su(Hw)attP1	3	w[1118]; P{y[+t7.7] w[+mC]=8XLexAop2-IVS-GAL80-WPRE}su(Hw)attP1
64306	Mef2	P{mb247-GAL80.T}2	2	y[1] w[67c23]; P{w[+m*]=mb247-GAL80.T}2

Stk #	Promoter	Symbol	Chr	Genotype
7016	alphaTub84B	P{tubP-GAL80[ts]}Sxl[9]	1	P{w[+mC]=tubP-GAL80[ts]}Sxl[9], w[*]/FM7c
7108	alphaTub84B	P{tubP-GAL80[ts]}10	2	w[*]; P{w[+mC]=tubP-GAL80[ts]}10; TM2/TM6B, Tb[1]
67058	alphaTub84B	P{tubP-GAL80[ts]}10	2	P{w[+mW.hs]=GawB}elav[C155]; P{y[+t7.7] w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40, P{w[+mC]=tubP-GAL80[ts]}10
67059	alphaTub84B	P{tubP-GAL80[ts]}10	2	w[1118] P{w[+mW.hs]=GawB}Bx[MS1096]; P{y[+t7.7] w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40, P{w[+mC]=tubP-GAL80[ts]}10
67060	alphaTub84B	P{tubP-GAL80[ts]}10	2	w[*]; P{y[+t7.7] w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40, P{w[+mC]=tubP-GAL80[ts]}10/CyO; P{w[+mW.hs]=GawB}pnr[MD237]/TM6B, Tb[1]
67061	alphaTub84B	P{tubP-GAL80[ts]}10	2	w[*]; P{y[+t7.7] w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40, P{w[+mC]=tubP-GAL80[ts]}10; P{w[+mC]=Lpp-GAL4.B}3/TM6B, Tb[1]
67062	alphaTub84B	P{tubP-GAL80[ts]}10	2	w[*]; P{y[+t7.7] w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40, P{w[+mC]=tubP-GAL80[ts]}10; P{GAL4-Mhc.W}MHC-82
67063	alphaTub84B	P{tubP-GAL80[ts]}10	2	w[*]; P{y[+t7.7] w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40, P{w[+mC]=tubP-GAL80[ts]}10; P{w[+mC]=GAL4-Mef2.R}R1
67064	alphaTub84B	P{tubP-GAL80[ts]}10	2	w[*]; P{y[+t7.7] w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40, P{w[+mC]=tubP-GAL80[ts]}10; P{w[+mW.hs]=GawB}bbg[C96]
67065	alphaTub84B	P{tubP-GAL80[ts]}10	2	w[*]; P{y[+t7.7] w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40, P{w[+mC]=tubP-GAL80[ts]}10; P{w[+mC]=tubP-GAL4}LL7/TM6B, Tb[1]
67066	alphaTub84B	P{tubP-GAL80[ts]}10	2	w[*]; P{y[+t7.7] w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40, P{w[+mC]=tubP-GAL80[ts]}10/CyO; P{w[+mW.hs]=GAL4-dpp.blk1}40C.6/TM6B, Tb[1]
92829	alphaTub84B	P{tubP-GAL80[ts]}10	2	w[*]; P{w[+mW.hs]=GawB}NP5130 P{w[+mC]=tubP-GAL80[ts]}10 P{w[+mC]=UAS-mCD8::GFP.L}LL5/CyO; P{y[+t7.7] w[+mC]=UAS-lin-28.G}attP2/TM6C, Sb[1] Tb[1]
92832	alphaTub84B	P{tubP-GAL80[ts]}10	2	w[*]; P{w[+mW.hs]=GawB}NP5130, P{w[+mC]=tubP-GAL80[ts]}10, P{w[+mC]=UAS-mCD8::GFP.L}LL5/CyO; TI{TI}InR[V5.rLUC]/TM6B, Tb[1]
7019	alphaTub84B	P{tubP-GAL80[ts]}20	2	w[*]; P{w[+mC]=tubP-GAL80[ts]}20; TM2/TM6B, Tb[1]
65406	alphaTub84B	P{tubP-GAL80[ts]}20	2	P{w[+mC]=Desat1-GAL4.E800}2M, P{w[+mC]=tubP-GAL80[ts]}20
91410	alphaTub84B	P{tubP-GAL80[ts]}20	2	w[*]; P{y[+t7.7] w[+mC]=mira(KDRT.stop)GAL4}attP40, P{w[+mC]=tubP-GAL80[ts]}20; P{y[+t7.7] w[+mC]=CG10116-KDR.PEST}attP2
91411	alphaTub84B	P{tubP-GAL80[ts]}20	2	M{RFP[3xP3.PB] w[+mC]=3xGBE-GAL80}ZH-2A w[*]; P{y[+t7.7] w[+mC]=mira(KDRT.stop)GAL4}attP40, P{w[+mC]=tubP-GAL80[ts]}20; P{y[+t7.7] w[+mC]=CG10116-KDR.PEST}attP2/TM6B, Tb[+]
600566	alphaTub84B	P{tubP-GAL80[ts]}20	2	w[*]; P{w[+mC]=UAS-Dcr-2.D}2; P{w[+mW.hs]=GawB}mirr[DE], P{w[+mC]=tubP-GAL80[ts]}20/TM6C, Sb[1] Tb[1]
600567	alphaTub84B	P{tubP-GAL80[ts]}20	2	w[*]; P{w[+mW.hs]=GawB}ptc[559.1], P{w[+mC]=UAS-GFP.nls}14, P{w[+mC]=tubP-GAL80[ts]}20
7017	alphaTub84B	P{tubP-GAL80[ts]}2	3	w[*]; P{w[+mC]=tubP-GAL80[ts]}2/TM2
65407	alphaTub84B	P{tubP-GAL80[ts]}2	3	P{w[+mC]=Desat1-GAL4.E800}4M, P{w[+mC]=tubP-GAL80[ts]}2
67047	alphaTub84B	P{tubP-GAL80[ts]}2	3	w[*]; P{y[+t7.7] w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40/CyO; P{w[+mW.hs]=GawB}Delta[05151-G], P{w[+mC]=tubP-GAL80[ts]}2/TM6B, Tb[1]
67067	alphaTub84B	P{tubP-GAL80[ts]}2	3	w[*]; P{w[+mW.hs]=GawB}Myo31DF[NP0001]/CyO; P{y[+t7.7] w[+mC]=UAS-3xFLAG.dCas9.VPR}attP2, P{w[+mC]=tubP-GAL80[ts]}2
67069	alphaTub84B	P{tubP-GAL80[ts]}2	3	w[*]; P{w[+mW.hs]=en2.4-GAL4}e16E, P{w[+mC]=UAS-2xEGFP}AH2/CyO; P{y[+t7.7] w[+mC]=UAS-3xFLAG.dCas9.VPR}attP2, P{w[+mC]=tubP-GAL80[ts]}2
67070	alphaTub84B	P{tubP-GAL80[ts]}2	3	w[*]; P{w[+mW.hs]=GawB}salm[LP39]/CyO; P{y[+t7.7] w[+mC]=UAS-3xFLAG.dCas9.VPR}attP2, P{w[+mC]=tubP-GAL80[ts]}2
67071	alphaTub84B	P{tubP-GAL80[ts]}2	3	w[*]; P{w[+mC]=bs-GAL4.Term}G1/CyO; P{y[+t7.7] w[+mC]=UAS-3xFLAG.dCas9.VPR}attP2, P{w[+mC]=tubP-GAL80[ts]}2
67072	alphaTub84B	P{tubP-GAL80[ts]}2	3	w[*]; P{w[+mW.hs]=GawB}NP5130 P{w[+mC]=UAS-GFP.U}2; P{y[+t7.7] w[+mC]=UAS-3xFLAG.dCas9.VPR}attP2, P{w[+mC]=tubP-GAL80[ts]}2
600374	alphaTub84B	P{tubP-GAL80[ts]}2	3	P{y[+t7.7] w[+mC]=UAS-mKir2.1.T2A.tdTomato}attP40; P{w[+mC]=tubP-GAL80[ts]}2
600375	alphaTub84B	P{tubP-GAL80[ts]}2	3	P{y[+t7.7] w[+mC]=UAS-mKcnj2.nc.T2A.tdTomato}attP40; P{w[+mC]=tubP-GAL80[ts]}2
604586	alphaTub84B	P{tubP-GAL80[ts]}2	3	w[*]; M{RFP[3xP3.PB] w[+mC]=nanos-GAL4.I}ZH-51C/CyO; P{w[+mC]=tubP-GAL80[ts]}2
7018	alphaTub84B	P{tubP-GAL80[ts]}ncd[GAL80ts-7]	3	w[*]; sna[Sco]/CyO; P{w[+mC]=tubP-GAL80[ts]}ncd[GAL80ts-7]
61669	alphaTub84B	P{tubP-GAL80[ts]}ncd[GAL80ts-7]	3	w[*]; S[1]/CyO; M{w[+mC]=UAS-p65AD::CaM}ZH-86Fb, P{w[+mC]=tubP-GAL80[ts]}ncd[GAL80ts-7]
90938	alphaTub84B	P{tubP-GAL80[ts]}ncd[GAL80ts-7]	3	w[*]; wg[Sp-1]/CyO; P{w[+mC]=tubP-GAL80[ts]}ncd[GAL80ts-7], P{w[+mC]=UAS-Dcr-2.D}10
91415	alphaTub84B	P{tubP-GAL80[ts]}ncd[GAL80ts-7]	3	w[]; P{w[+m]=Uro-GAL4.T}2; P{w[+mC]=tubP-GAL80[ts]}ncd[GAL80ts-7]
92351	alphaTub84B	P{tubP-GAL80[ts]}ncd[GAL80ts-7]	3	w[*]; P{w[+mW.hs]=en2.4-GAL4}e16E, P{w[+mC]=UAS-RFP.W}2/CyO, P{w[+mC]=GAL4-Kr.C}DC3, P{w[+mC]=UAS-GFP.S65T}DC7; P{y[+t7.7] w[+mC]=Ubi-LCasExpress}attP2 P{w[+mC]=tubP-GAL80[ts]}ncd[GAL80ts-7]/TM6B, Tb[1]

Stk #	Promoter	Symbol	Chr	Genotype
38879	alphaTub84B	P{alphaTub84B(FRT.GAL80)}1	1	P{w[+mC]=alphaTub84B(FRT.GAL80)}1, w[*]; Bl[1]/CyO; TM2/TM6B, Tb[1]
90899	alphaTub84B	P{alphaTub84B(FRT.GAL80)}1	1	P{w[+mC]=alphaTub84B(FRT.GAL80)}1, w[*]; P{w[+mC]=tub-QS.P}4C P{ry[+t7.2]=neoFRT}40A/CyO; P{y[+t7.7] w[+mC]=nSyb-QF2w.P}attP2, P{w[+mC]=QUAS-mCD8-GFP.P}5B
38880	alphaTub84B	P{alphaTub84B(FRT.GAL80)}2	2	w[*]; P{w[+mC]=alphaTub84B(FRT.GAL80)}2/CyO; TM2/TM6B, Tb[1]
58399	alphaTub84B	P{alphaTub84B(FRT.GAL80)}2	2	w[*]; P{w[+mC]=alphaTub84B(FRT.GAL80)}2, TI{TI}brp[SNAPf-tag]/CyO; P{y[+t7.7] w[+mC]=UAS-myr-Halo}attP2
38878	alphaTub84B	P{tubP(FRT.stop)GAL80}2	2	w[*]; P{w[+mC]=tubP(FRT.stop)GAL80}2; MKRS/TM6B, Tb[+]
38881	alphaTub84B	P{alphaTub84B(FRT.GAL80)}3	3	w[*]; Bl[1]/CyO; P{w[+mC]=alphaTub84B(FRT.GAL80)}3
39213	alphaTub84B	P{tubP(FRT.stop)GAL80}3	3	w[*]; wg[Sp-1]/CyO; P{w[+mC]=tubP(FRT.stop)GAL80}3
62104	alphaTub84B	PBac{tubP-(B3RT.GAL80)}VK00027	3	w[1118]; PBac{y[+mDint2] w[+mC]=tubP-(B3RT.GAL80)}VK00027
62103	alphaTub84B	PBac{tubP-(FRT.GAL80)}VK00027	3	w[1118]; PBac{y[+mDint2] w[+mC]=tubP-(FRT.GAL80)}VK00027
62105	alphaTub84B	PBac{tubP-(KDRT.GAL80)}VK00027	3	w[1118]; PBac{y[+mDint2] w[+mC]=tubP-(KDRT.GAL80)}VK00027

Related links GAL4 UAS FRT

GAL80 lines

Stocks Carrying GAL80

Stocks carrying GAL80[ts] (temperature sensitive GAL80)

Stocks carrying GAL80 that can be controlled by a "flippable" FRT, B3RT, or KDRT cassette

Bloomington Drosophila Stock Center social media channels