FLP/FRT and fluorescent markers can be used to generate mitotic clones in the eye with normal and mutant photoreceptor cells expressing different fluorescent markers. The method was described in:
Gambis, A., Dourlen, P., Steller, H., Mollereau, B. (2011). Two-color in vivo imaging of photoreceptor apoptosis and development in Drosophila. Dev. Biol. 351(1): 128--134.
| Stock # | FRT | tdTomato | Genotype | Chr_arm |
|---|---|---|---|---|
| 43345 | P{neoFRT}40A | P{ninaE-tdTomato-ninaC}2L | P{ry[+t7.2]=rh1-GAL4}1, P{ry[+t7.2]=ey-FLP.N}2; P{w[+mC]=ninaE-tdTomato-ninaC}2L P{ry[+t7.2]=neoFRT}40A; P{w[+mC]=UAS-GFP-ninaC}3 | 2L |
| 43346 | P{neoFRT}42D | P{ninaE-tdTomato-ninaC}2R | P{ry[+t7.2]=rh1-GAL4}1, P{ry[+t7.2]=ey-FLP.N}2; P{ry[+t7.2]=neoFRT}42D P{w[+mC]=ninaE-tdTomato-ninaC}2R; P{w[+mC]=UAS-GFP-ninaC}3 | 2R |
| 43347 | P{neoFRT}80B | P{ninaE-tdTomato-ninaC}3L | P{ry[+t7.2]=rh1-GAL4}1, P{ry[+t7.2]=ey-FLP.N}2; P{w[+mC]=UAS-GFP-ninaC}2; P{w[+mC]=ninaE-tdTomato-ninaC}3L P{ry[+t7.2]=neoFRT}80B/TM6B, Tb[1] | 3L |
| 43348 | P{neoFRT}82B | P{ninaE-tdTomato-ninaC}3R | P{ry[+t7.2]=rh1-GAL4}1, P{ry[+t7.2]=ey-FLP.N}2; P{w[+mC]=UAS-GFP-ninaC}2; P{ry[+t7.2]=neoFRT}82B P{w[+mC]=ninaE-tdTomato-ninaC}3R/TM6B, Tb[1] | 3R |
Related links Clonal/lineage analysis
