The following P{hsp26-pt-T} insertions were chosen by Sally Elgin to provide position-effect variegation patterns of miniwhite characteristic of centric, telomeric, Y and fourth chromosome heterochromatin.
P{hsp26-pt-T} carries Hsp26 regulatory sequences driving expression of a plant cDNA sequence and Hsp70 regulatory sequences driving expression of miniwhite.
Representative expression patterns of the eyes in the stocks below can be downloaded here (beware it is a big file).
| Stock # | Insertion | Position | Pattern | Genotype |
|---|---|---|---|---|
| 44259 | P{hsp26-pt-T}39C-5 | 2L Telomere Associated Sequence (TAS) repeats | Medium to dark orange variegated eye pigmentation with darker spots. | y[1] w[67c23]; P{w[+mW.hs]=hsp26-pt-T}39C-5 al[1] |
| 44261 | P{hsp26-pt-T}39C-62 | 3R Telomere Associated Sequence (TAS) repeats | Nearly normal eye pigmentation with subtle darker variegation. | y[1] w[67c23]; P{w[+mW.hs]=hsp26-pt-T}39C-62 |
| 84108 | P{hsp26-pt-T}118E-10 | 4th chromosome centric heterochromatin | Light to medium, variegated eye pigmentation. | y[1] w[67c23]; P{w[+mW.hs]=hsp26-pt-T}118E-10 |
| 84105 | P{hsp26-pt-T}118E-12 | 3R centric heterochromatin | Mostly white eyes with a few lightly pigmented facets. | y[1] w[67c23]; P{w[+mW.hs]=hsp26-pt-T}118E-12 |
| 84106 | P{hsp26-pt-T}118E-16 | 3R Telomere-Associated Sequences | Nearly wild type eye pigmentation. | y[1] w[67c23]; P{w[+mW.hs]=hsp26-pt-T}118E-16 |
| 84091 | P{hsp26-pt-T}118E-25 | X centric heterochromatin | Wide range of variegated eye pigmentation; generally medium to dark. | y[1] w[67c23] P{w[+mW.hs]=hsp26-pt-T}118E-25 |
| 84110 | P{hsp26-pt-T}39C-12 | 4th chromosome sequence | Light to medium, variegated eye pigmentation. | y[1] w[67c23]; P{w[+mW.hs]=hsp26-pt-T}39C-12 |
| 84100 | P{hsp26-pt-T}39C-2 | 2R centric heterochromatin | Light to medium, variegated eye pigmentation. May be segregating SM6a. | y[1] w[67c23]; P{w[+mW.hs]=hsp26-pt-T}39C-2 |
| 84098 | P{hsp26-pt-T}39C-3 | 2L centric heterochromatin | Wide range of variegated eye pigmentation. | y[1] w[67c23]; P{w[+mW.hs]=hsp26-pt-T}39C-3 |
| 84099 | P{hsp26-pt-T}39C-4 | 2L centric heterochromatin | Mostly white eyes with a few pigmented facets. | y[1] w[67c23]; P{w[+mW.hs]=hsp26-pt-T}39C-4 |
| 84101 | P{hsp26-pt-T}39C-50 | 2R Telomere-Associated Sequences | Nearly wild type eye pigmentation with darker spots. | y[1] w[67c23]; P{w[+mW.hs]=hsp26-pt-T}39C-50 |
| 84102 | P{hsp26-pt-T}39C-58 | 2R Telomere-Associated Sequences | Nearly wild type eye pigmentation with darker spots. | y[1] w[67c23]; P{w[+mW.hs]=hsp26-pt-T}39C-58 |
| 84092 | P{hsp26-pt-T}39C-66 | Het-A element at YS telomere | Mostly white eyes with pigmented splotches. | y[1] w[67c23]/P{w[+mW.hs]=hsp26-pt-T}39C-66 |
| 84094 | P{hsp26-pt-T}7-M143 | TART-element at YS telomere | White eyes with a few pigmented facets. | w[*]/P{w[+mW.hs]=hsp26-pt-T}7-M143; sv[spa-pol] |
| 84093 | P{hsp26-pt-T}8-M112 | Het-A element at YS telomere | Mostly white eyes with pigmented splotches. | w[*]/P{w[+mW.hs]=hsp26-pt-T}8-M112 |
| 84095 | P{hsp26-pt-T}8-M114 | TART-element at YS telomere | Mostly white eyes with pigmented splotches. | w[*]/P{w[+mW.hs]=hsp26-pt-T}8-M114 |
| 84096 | P{hsp26-pt-T}8-M76 | TART-element at YS telomere | Mostly white or pale orange eyes with pigmented splotches. | w[*]/P{w[+mW.hs]=hsp26-pt-T}8-M76 |
| 84109 | P{hsp26-pt-T}Abcd1[39C-34] | 4th chromosome sequence | Medium to dark, variegated eye pigmentation. | y[1] w[67c23]; P{w[+mW.hs]=hsp26-pt-T}Abcd1[39C-34] |
| 84111 | P{hsp26-pt-T}CaMKII[118E-9] | 4th chromosome sequence | Light to medium, variegated eye pigmentation. | y[1] w[67c23]; P{w[+mW.hs]=hsp26-pt-T}CaMKII[118E-9] |
| 84107 | P{hsp26-pt-T}Dyrk3[118E-15] | nonrepetitive fourth chromosome sequence | Medium to dark, variegated eye pigmentation. | y[1] w[67c23]; P{w[+mW.hs]=hsp26-pt-T}Dyrk3[118E-15] |
The following stocks contain the nonvariegating P{hsp26-pt-T} insertions mobilized to generate the insertions above.
| Stock # | Insertion | Position | Genotype |
|---|
Protocols for mapping new insertions are given in Cryderman et al. (1999). The primers for inverse or direct PCR shown in the figure above are 1, 5′-AACTCGAGGCCTCGAGGT-3′; 2, 5′-GACGAAATGACCCACTCGG-3′; 3, 5′-GCTTCGGCTATCGACGGGACCACC-3′.
