This medium was developed by Brent and Oster (1974) and was the medium used by the Mid-America Stock Center for many years. The substitution of dextrose for sucrose is intended to reduce the consequences of Leuconostoc infection of cultures by preventing the bacterium for producing its slime coat. This recipe has not been used at Bloomington.
Ingredients
| Water | 15.4 | liters |
| Agar* | 80 | grams |
| Brewer's yeast | 280 | grams |
| Cornmeal | 1,400 | grams |
| Dextrose | 2,270 | grams |
| 10% p-Hydroxy-benzoic acid methyl ester in 95% ethanol | 250 | milliliters |
| 1.5% benzyl benzoate in 95% ethanol | 350 | milliliters |
| * adjust agar concentration according to the gel strength of your agar | ||