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Stocks for detecting viral infections

Ekstrom and Hultmark (2016) recently developed the Munin system for detecting viral infections in Drosophila. The system consists of GAL4 tethered to the cell membrane via a linker carrying a cleavage site recognized by a specific viral protease. In the presence of virus, GAL4 is liberated from the membrane and can activate transcription from a UAS-driven reporter.

schematic of Munin system
Virus detected Stk # Genotype
Drosophila C virus 65686 y[1] w[1118]; PBac{y[+mDint2] w[+mC]=Tub-Munin.C-virus}VK00013
Nora virus 65685 y[1] w[1118]; PBac{y[+mDint2] w[+mC]=Tub-Munin.Nora-virus}VK00013