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Redox sensors

Redox-sensitive GFP (roGFP) fluoresces with different intensities depending on the local redox conditions in the cell. It can be fused to glutaredoxin (Grx1/GLRX) to monitor glutathione redox potentials or it can be fused to oxidant receptor peroxidase (Orp1/HYR1) to monitor hydrogen peroxide levels.

Redox sensor illustration originating in Albrecht et al. (2011), In Vivo Mapping of Hydrogen Peroxide and Oxidized Glutathione Reveals Chemical and Regional Specificity of Redox Homeostasis. Cell Metabolism 14: 819-829

Moreover, these fusion proteins can be localized to the cytoplasm or mitochondria to sense redox conditions in different cellular compartments.

These methods were described in Albrecht et al. (2011). In Vivo Mapping of Hydrogen Peroxide and Oxidized Glutathione Reveals Chemical and Regional Specificity of Redox Homeostasis.

Stk # Localization Controlled by: Sensor Genotype
67668 cytoplasmic alphaTub84B glutaredoxin w[1118]; P{w[+mC]=tub-cyto-Grx1-roGFP2}4
67670 cytoplasmic alphaTub84B oxidant receptor peroxidase w[1118]; P{w[+mC]=tub-cyto-roGFP2-Orp1}1
67671 cytoplasmic alphaTub84B oxidant receptor peroxidase w[1118]; P{w[+mC]=tub-cyto-roGFP2-Orp1}7
67662 cytoplasmic UAS glutaredoxin w[1118]; P{w[+mC]=UAS-cyto-Grx1-roGFP2}13
67663 cytoplasmic UAS glutaredoxin w[1118]; P{w[+mC]=UAS-cyto-Grx1-roGFP2}13; P{w[+mC]=tubP-GAL4}LL7/TM3, Ser[1]
67666 cytoplasmic UAS oxidant receptor peroxidase w[1118]; P{w[+mC]=UAS-cyto-roGFP2-Orp1}1
67669 mitochrondrial alphaTub84B glutaredoxin w[1118]; P{w[+mC]=tub-mito-roGFP2-Grx1}6
67672 mitochrondrial alphaTub84B oxidant receptor peroxidase w[1118]; P{w[+mC]=tub-mito-roGFP2-Orp1}3
67673 mitochrondrial alphaTub84B oxidant receptor peroxidase w[1118]; P{w[+mC]=tub-mito-roGFP2-Orp1}6
67664 mitochrondrial UAS glutaredoxin w[1118]; P{w[+mC]=UAS-mito-roGFP2-Grx1}9
67665 mitochrondrial UAS glutaredoxin w[1118]; P{w[+mC]=UAS-mito-roGFP2-Grx1}9; P{w[+mC]=tubP-GAL4}LL7/TM3, Ser[1]
67667 mitochrondrial UAS oxidant receptor peroxidase w[1118]; P{w[+mC]=UAS-mito-roGFP2-Orp1}10