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InSITE stocks
The InSITE (Integrase Swappable In Vivo Targeting Element) system allows for conversion of a GAL4 enhancer trap into one containing lexA, QF, or a number of other transcriptional regulators using simple genetic crosses. Initially, GAL4-bearing enhancer trap lines are isolated by either P- or piggyBac-based element transposition (two suitable “ammunition” lines that can be used as transposition sources are available). In subsequent crosses, the GAL4 cassette in these lines can then be swapped for “donor” cassettes encoding other genetic effectors.

The InSITE system is described in Gohl et al. 2011. “A versatile in vivo system for directed dissection of gene expression patterns.” Nature Meth. 8(3):231-237. FBrf0213241

Gohl et al. Supplementary Figure 5 containing crossing schemes for doing swaps can be found here.

A list of currently available InSITE GAL4 lines can be found here.
InSITE swap overview
In the presence of an enhancer trap insertion (the “target”), the donor insertion, and FLP and phiC31 recombinases, FLP recombinase will FLP-out the FRT-flanked attB-bearing donor and phiC31 will put the freed donor into the attP site of the enhancer trap target. At this step, the target site (which now carries both GAL4 and the donor gene) contains a FRT site which can be used for generating deletions. Subsequent steps using Cre recombinase complete the swap by removing the GAL4 (this step also removes the FRT).
Donor cassettes (see below for available insertion stocks):
P{ID.GAL4AD} GAL4-activator domain fusion gene for the split GAL4 system
P{ID.GAL4DBD} GAL4-DNA binding domain fusion gene for the split GAL4 system
P{ID.GAL80} GAL80 (GAL4 inhibitor)
P{ID.LexA} LexA transcriptional activator
P{ID.QF} QF transcriptional activator
P{ID.VP16AD} GAL4-VP16 activator domain fusion gene for the split GAL4 system

The BDSC distributes the following stocks for use in the InSITE system:
Type Stk# Symbol Chr Comments
Ammunition stocks for generating new enhancer trap lines
PBac ammo 33215 PBac{IT.GAL4}0315 1 Useful as a GAL4-containing PBac-based source for transposition.
P element ammo 33598 P{IT.GAL4}A134.3 1 Useful as a GAL4-containing P-element-based source for transposition.
Tool stocks for swapping cassettes in vivo
FLP and phiC31 33216 P{hsFLP}122 M{vas-int.B}ZH-2A 1 Expresses FLP under the control of heatshock and phiC31 integrase under the control of vasa.
Cre recombinase 766 P{Crey}1b 1 Expresses Cre in both germline and somatic tissue. y+ marked insert on X with sna[Sco]/CyO in the background.
Cre recombinase 851 P{Crey}1b 1 Expresses Cre in both germline and somatic tissue. y+ marked insert on X with D[*]/TM3, Sb[1] in the background.
Cre recombinase 1092 P{Crew}DH1 2 Expresses Cre in both germline and somatic tissue. w+ marked insert on CyO.
Cre recombinase 1501 P{Crew}DH2 3 Expresses Cre in both germline and somatic tissue. w+ marked insert on TM6B, Tb[1].
ey-FLP 5580 P{ey-FLP.N}2 1 Expresses FLP in the eye. Useful for preventing the selection of unexcised donor lines during swap crosses
Type Stk# Symbol Chr Insert site (cyto) Insert site (r6 seq) Orientation
Donor stocks
GAL4AD donor 33240 P{ID.GAL4AD}G18.2 2 53D11 2R:16828854..16828885 minus
GAL4AD donor 33241 P{ID.GAL4AD}dnr1[G39.2L] 2 58E9 2R:22570273..22570273 plus
GAL4AD donor 33239 P{ID.GAL4AD}CR31044[G12.1] 3 99A1 3R:29218454..29218515 plus
GAL4AD donor 33238 P{ID.GAL4AD}CstF-50[G10.1] 3 100D1 3R:31736875..31736906 minus
GAL4DBD donor 33227 P{ID.GAL4DBD}F17.1 2 31A1-31A2 2L:10055469..10057038 plus
GAL4DBD donor 33228 P{ID.GAL4DBD}MESR3[F29.2] 2 36F7 2L:18640701..18640701 plus
GAL4DBD donor 33229 P{ID.GAL4DBD}Edc3[F32.1] 3 74D2 3L:17529786..17529848 minus
GAL4DBD donor 33226 P{ID.GAL4DBD}Kdm2[F11.1] 3 85C3 3R:9056805..9056805 minus
GAL80 donor 33225 P{ID.GAL80}MFS3[E22.1] 2 21F1 2L:1143723..1143739 plus
GAL80 donor 33222 P{ID.GAL80}Pde8[E6.1] 2 59F5 2R:23666987..23666987 minus
GAL80 donor 33223 P{ID.GAL80}E10.1 3 84F6 3R:8250470..8250470 plus
GAL80 donor 33224 P{ID.GAL80}tok[E17.1] 3 96A18 3R:24720014..24720023 minus
LexA donor 33237 P{ID.LexA}l(2)09851[L44.2] 2 41F9 2R:5754525..5754671 minus
LexA donor 33235 P{ID.LexA}L19.2L 2 53C4 2R:16386495..16386511 plus
LexA donor 33236 P{ID.LexA}L34.2L 3 66B4 3L:8063834..8063962 minus
LexA donor 33234 P{ID.LexA}CTPsyn[L4.3] 3 71B4 3L:15107476..15107483 plus
QF donor 33232 P{ID.QF}Cul-3[Q12A] 2 35D1 2L:15271465..15271465 minus
QF donor 33231 P{ID.QF}NaCP60E[Q10B] 2 60E5 2R:24903641..24903641 minus
QF donor 33233 P{ID.QF}Q33A 3 65A9 3L:6218016..6218084 plus
QF donor 33230 P{ID.QF}CG7370[Q2A] 3 78F1 3L:21770660..21770660 plus
VP16AD donor 33217 P{ID.VP16AD}D33.1 2
VP16AD donor 33219 P{ID.VP16AD}D38.3 2 47D6 2R:11173765..11173776 minus
VP16AD donor 33221 P{ID.VP16AD}CG5742[D40.1] 2 55B9 2R:18148692..18148692 plus
VP16AD donor 33218 P{ID.VP16AD}D37.1 3 77C2 3L:20410610..20410610 plus
VP16AD donor 33220 P{ID.VP16AD}rdx[D39L.2] 3 88A4 3R:14018548..14018548 plus