Targeted Gene Knock-Out Stocks
Updated July 24, 2015
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These stocks constitute the gene replacement kit for creating targeted knock-outs from the Golic lab (see Rong and Golic, 2000 and Rong et al. 2003).
Stock #
1 w[1118]; P{70FLP}10
2 y[1] w[*]; P{70FLP}11 P{70I-SceI}2B noc[Sco]/CyO, S[2]
3 y[1] w[*]; P{70FLP}23 P{70I-SceI}4A/TM6
4 P{hs-I-CreI.R}2A, v[1]; ry[506]
5 w[1118]; P{hs-I-CreI.R}1A Sb[1]/TM6
6 y[1] w[*]; P{y-donor}1B Sb[1]/TM6
7 w[1118]; P{w8,I-site}7
These stocks are similar to stocks 6934 and 6935, but they allow heat shock killing of unwanted progeny classes in knock-out screens. Their use is described in Huang et al. (2008).
Stock #
1 y[1] w[*]/Dp(2;Y)G, P{hs-hid}Y; P{70FLP}11 P{70I-SceI}2B noc[Sco]/CyO, P{hs-hid}4
2 y[1] w[*]/Dp(2;Y)G, P{hs-hid}Y; P{70FLP}23 P{70I-SceI}4A/TM3, P{hs-hid}14, Sb[1]