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Stocks carrying X-linked lethals from the Bellen lab
This collection of X Chr lethals was generated by Hugo Bellen's lab as part of a forward genetic screen utilizing low concentrations of EMS to minimize lethal hits per chromosome. Acquisition of this collection is ongoing. More stocks will be added as they become available.

Briefly, y[1] w[*] P{neoFRT}19A chromosomes were mutagenized and lethal mutations were isolated. Selection was based on phenotypic characterization of homozygous mutant tissues in two types of clones electroretinograms of ey-FLP eye clones and morphological observation of wings and thoraces of Ubx-FLP clones. Complementation tests, duplication mapping and P[acman] mapping were performed, and mutations in selected cases were determined by PCR and Sanger sequencing and/or whole genome sequencing.

For more information see:
Yamamoto et al., 2014
Haelterman et al., 2014


All mutations are balanced over FM7c, Kr-GAL4 UAS-GFP sn+.
  Stk # Allele Mutation Comment
1 57049 AMPKalpha[A] S188L
2 57050 AMPKalpha[B] H149L
3 57051 AP-1gamma[B] Splice acceptor X:9,112,162 (G -> A)
4 57052 AP-1gamma[D] L133P
5 57053 arm[E] S2C
6 57054 arm[F] Q84stop
7 52315 Arp2[A] W89stop
8 57055 ATP7[B] G579R
9 57056 ATP7[O] R355stop
10 57057 ben[A] W129stop
11 57058 ben[B] P120L
12 57060 beta-Spec[A] not assessed
13 57059 beta-Spec[C] W642stop
14 57061 betaCOP[A] S570F
15 57062 Brms1[A] K188stop
16 57063 Brms1[B] M1I and A235T
17 52316 br[A] no mutation found by WGS
18 52317 br[B] C665R Some or all homozygotes die as pupae.
19 67175 cac[F] Harbors an EMS-induced E989K mutation in the cac gene.
20 67176 cac[J] Harbors an EMS-induced L284term mutation in the cac gene.
21 57064 cac[K] W623stop
22 52318 CDC45L[A] Q540stop Some or all homozygotes die as first or second instar larvae.
23 57067 Cdk7[A] E68K
24 57068 Cdk7[B] G200D
25 52319 CG11417[A] R103stop
26 65428 CG12991[A] A245T
27 52322 CG13365[A] V47D
28 52323 CG13365[B]
29 67107 CG14442[A] T755P
30 52324 CG15208[A] L318F
31 52325 CG1703[A] Q641stop
32 52328 CG17776[A] Y21stop
33 57074 CG17829[B] H253R
34 57076 CG2025[A] W369stop
35 57077 CG2025[B] Q376stop
36 57078 CG2918[B] Q619stop
37 52330 CG3149[A] W535stop
38 54258 CG3149[B] A410V
39 52331 CG32649[A] E623K
40 52332 CG32649[B] R629C
41 67108 CG32694[A] point mutation in 5' UTR
42 67109 CG32694[C] Q91@
43 57079 CG32795[A] A196V
44 52333 CG34401[A] W946stop
45 52334 CG34401[B] T295M
46 52336 CG3704[A] V124E
47 52337 CG3704[B] E300stop
48 52338 CG3857[A] L467V
49 64462 CG4078[B] V11E
50 52339 CG42237[A] E361K
51 52340 CG42749[A] Q27X
52 67110 CG4407[A] D128N
53 67111 CG4407[B] V205G
54 52342 CG7065[A] K327stop Some or all homozygotes die as pupae.
55 57081 CG7358[A] N782S
56 57082 CG7358[C] L730stop
57 52343 CG8184[B] T1107I
58 57083 CG9650[A] splice donor X:7,233,231 (T -> A)
59 67112 Chc[B] G314S
60 57084 CkIalpha[A] L141M
61 64459 CkIalpha[B] G148S
62 67113 CkIIbeta[A] 617bp deletion
63 57085 comt[A] L257Q
64 52345 COQ7[B] W90stop
65 67114 Cp7Fb[A] V3G
66 67115 Cp7Fb[B] I312N
67 52346 Crag[A] C1372S
68 52347 Cyp4d2[A] K350X
69 52348 DAAM[A] D360V
70 52349 deltaCOP[A] P395S
71 57086 dlg1[A] Q551stop
72 57087 dlg1[B] splice donor X:11,395,793 (T -> A)
73 67116 Dlic[A] Q1090@
74 67117 Dlic[B] R1021@
75 52350 dor[C] Q208stop
76 54795 dsh[B] No mutation found by WGS
77 52351 dsh[C]
78 67118 Dsor1[B] N4D
79 57088 dwg[A] C363S
80 57089 dwg[B] H411L
81 52352 Efr[A] M216K
82 52353 egh[A] D241N
83 52354 egh[B] V333E
84 67144 Ehbp1[A28] Harbors an AG to AA mutation in the splice acceptor of the 4th intron of Ehbp1(RB isoform).
85 67143 Ehbp1[O4] Harbors a 102bp deletion within?Ehbp1.
86 57090 eIF2Bepsilon[A] Y534stop
87 52344 eIF3g1[A] K216stop
88 52355 elav[A] Some or all homozygotes die as embryos.
89 52356 elav[B] Q122stop Some or all homozygotes die as embryos.
90 52357 ewg[A] Q544K (PE isoform) Some or all homozygotes die as first instar larvae.
91 52358 ewg[B] Q166stop Some or all homozygotes die as embryos or first instar larvae.
92 67162 fh[1] Harbors an EMS induced S136R mutation in the fh gene.
93 67161 fh[1] Harbors an EMS induced S136R mutation in the fh gene.
94 67119 fliI[A] A715V
95 57091 flw[E] K156stop
96 67120 fs(1)h[B] W211@
97 57092 Fur2[A] splice donor X:16,375,861 (T -> A)
98 52384 fzr[A]
99 52385 fzr[B]
100 57073 GCS1[A] G836D
101 57072 GCS1[E] Y761N
102 52359 Gtp-bp[B] V439D
103 52314 HisRS[A] D160V
104 52360 Hlc[A] G58D
105 52361 hop[C] Q115stop
106 67121 Hr4[A] Q867@
107 67122 Hr4[C] W728@
108 52362 Hsc70-3[A] R181H
109 52363 Inx2[B] Q194stop
110 52364 kdn[A] W370stop (PB isoform) Some or all homozygotes die as first instar larvae.
111 52365 kdn[B] K393stop (PB isoform) Some or all homozygotes die as first instar larvae.
112 52366 l(1)1Bi[A] Q860stop
113 52367 l(1)1Bi[B] L285stop
114 57095 l(1)G0156[A] Splice donor X:19,519,694 (A -> T)
115 52368 l(1)G0255[A] V141M
116 52369 l(1)G0255[B] S172F
117 52370 l(1)G0334[A] G126E (PA isoform)
118 52371 l(1)G0334[B] S296F (PA isoform)
119 67166 Lrpprc2[A] Harbors an EMS-induced W151term mutation in the Lrpprc2 gene.
120 67168 Lrpprc2[A] Harbors an EMS-induced W151term mutation in the Lrpprc2 gene.
121 67167 Lrpprc2[E] Harbors an EMS-induced Q351term mutation in the Lrpprc2 gene.
122 57069 Lrpprc2[H] K313stop
123 67154 Marf[B] Harbors an EMS-induced I221N mutation in the Marf gene.
124 67155 Marf[E] Harbors an EMS-induced V695E mutation in the Marf gene.
125 57097 Marf[I] E382K
126 57096 Marf[J] A207V
127 57098 Mcm6[A] Q510stop
128 57099 Mcm6[B] L362P
129 52372 mew[A] R165stop
130 57070 mldr[B] Y121D
131 57071 mldr[C] W465R
132 57100 mRNA-cap[A] Y346stop
133 57101 mRNA-cap[B] V377D
134 52373 mRpL38[A] Q88stop
135 52374 mRpL38[B] splice acceptor X:14121229 G->A
136 57102 mRpS25[A] splice donor X:14,582,756 (C -> T)
137 57103 mRpS30[A] splice donor X:15,786,631 (T -> A)
138 52375 mst[A] V216E
139 52320 MTPAP[C] Q47stop Some or all homozygotes die as pupae.
140 52321 MTPAP[D] Q160stop Some or all homozygotes die as larvae.
141 52376 mus101[A] Q525stop
142 57104 mxc[F] Q615stop
143 52377 Myb[A] G111stop
144 52378 Myb[B] splice donor X:15749822
145 52335 ND-B16.6[A] E82V
146 57075 ND-MNLL[A] stop57L
147 57105 nej[B] Y1001N
148 52379 Nmd3[A] V4516E
149 67123 nonC[A] A928V
150 67124 Nup93-1[A] K239@
151 57106 oc[A] Q469stop
152 57107 oc[B] splice acceptor X:8,633,964 (T -> A)
153 52380 ogre[A] V93E
154 57108 para[A] N343I
155 57109 para[B] V1424E
156 67125 parvin[A] Q45@
157 67126 parvin[B] Q312@
158 57110 pck[A] P50L
159 57111 Pgd[A.B] R286stop
160 57112 PI4KIIIalpha[C] W879stop
161 57113 por[B] R135stop
162 57115 pot[B] not assessed
163 57114 pot[D] K551stop
164 57116 PpV[A] W203R
165 57117 PpV[C] I117F
166 52383 Prosalpha4[A] G36R
167 57119 Psf3[A] G105D
168 57118 Psf3[B] Q141stop
169 52381 Raf[A] H546Y
170 52382 Raf[B] L163R
171 57120 Rbcn-3A[A] Q41stop
172 57121 Rbcn-3A[B] W291stop
173 57122 Rbcn-3B[B] R1294stop
174 64460 Rbcn-3B[C] W1254X
175 57123 Rbf[A] K458stop
176 57124 Rbf[C] splice acceptor X:799,014 (C -> T)
177 52386 Rhp[A] M1K
178 57126 RpII215[A] not assessed
179 57125 RpII215[B] V785E
180 52387 RpS5a[A] Y80stop
181 57127 Sas10[A] Q177stop
182 57128 schlank[A] S344P
183 52388 scu[A] Q159stop Some or all homozygotes die as pupae.
184 52390 Sec16[A] P1926S
185 57129 sgg[A] P223L
186 64461 sgg[B] V68E
187 52391 shi[A] K28stop
188 52392 shi[N] P221L
189 52329 Shmt[A] M281R
190 52393 sicily[C] deletion removing promoter and first two exons
191 52394 sicily[E] Q133stop
192 57130 SkpA[A] L22stop
193 57065 SMC3[A] K575stop
194 57066 SMC3[B] Q146stop
195 57131 Smox[A] Q313stop
196 57133 Smr[C] Q1655stop
197 57132 Smr[L] Q2002stop
198 57134 Sp1[A] C197S
199 57135 Sp1[B] Q8stop
200 52395 Stim[A] W202stop
201 52396 Stim[B] K426stop
202 57136 svr[A] S950T
203 52397 tay[A] R990stop
204 57094 temp[A] K14stop
205 57093 temp[H] Q190stop
206 52398 TH1[A] R149stop Some or all homozygotes die as second instar larvae.
207 52399 TH1[B] splice donor X:16284495 C->T
208 52400 tko[A] L85H Some or all homozygotes die as third instar larvae.
209 52401 tko[B] Q6stop Some or all homozygotes die as second instar larvae.
210 57137 trr[A] R1477stop
211 57138 trr[B] K512stop
212 66578 Uba5[A] V178E Some or all homozygotes die as pupae.
213 66348 Uba5[B] K298stop Some or all homozygotes die as pupae.
214 67185 Ubr3[A] Harbors an EMS-induced F949L mutation.
215 67186 Ubr3[B] Harbors an EMS-induced L788term mutation.
216 52402 Upf1[A] I486N, H1002P, V1051G and A1106G
217 52403 Upf1[B] W120stop
218 52404 Usf[A] Y248N
219 57080 Usp16-45[B] splice donor X:5,423,751 (G -> A)
220 67127 Usp7[B] Y474N
221 57139 vnd[A] splice acceptor X:593,646 (A -> T)
222 57140 Vps26[B] K264stop
223 57141 Vps26[C] V267D
224 52405 wapl[A] splice acceptor X:2045880 (C->T) Some or all homozygotes die as second instar larvae.
225 52406 wapl[C] Y842stop
226 67177 wcy[A] Harbors an EMS-induced Q253L (PA isoform) mutation in the wcy gene.
227 67178 wcy[B] Harbors a deletion removing amino acids 590-760 within the wcy gene.
228 67179 wcy[C] Harbors an EMS-induced Q513term (PA isoform) mutation in the wcy gene.
229 52407 wds[A] G132D
230 52408 wus[A] Q307stop and I375V
231 52341 xit[A] W350R
232 57142 Ykt6[C] Q62R
233 52409 Zpr1[A] C46S
234 52410 Zpr1[B] splice acceptor X:9055222 T->A