TRiP Toolbox Stocks
Updated October 30, 2009
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The Transgenic RNAi Project (TRiP) is producing UAS-RNAi constructs for hundreds of genes. The stocks listed below are used by TRiP to generate RNAi transgenic lines and to assess their ability to knock down gene expression. Many TRiP stocks with UAS-RNAi insertions are available from Bloomington.
  Stock # Symbol Components
1 25709 P{nos-phiC31\int.NLS}X; P{CaryP}attP40 nanos promoter driving phiC31 integrase; 2nd chromosome target site
2 25710 P{nos-phiC31\int.NLS}X; P{CaryP}attP2 nanos promoter driving phiC31 integrase; 3rd chromosome target site
  Stock # Symbol Expresses dsRNA and Dcr2 in
1 25706 P{GawB}Bx[MS1096]; P{UAS-Dcr-2.D}2 Dorsal wing disc
2 25707 P{UAS-Dcr-2.D}1; P{GAL4-twi.2xPE}1 Ventral stripe at cellular blastoderm
3 25708 P{UAS-Dcr-2.D}1; P{Act5C-GAL4}25FO1 All cells
4 25750 P{GawB}elav[C155]; P{UAS-Dcr-2.D}2 Nervous system
5 25751 P{UAS-Dcr-2.D}1; P{GAL4-nos.NGT}40 Female germ line
6 25752 P{UAS-Dcr-2.D}1; P{en2.4-GAL4}e16E engrailed domain
7 25753 P{UAS-Dcr-2.D}1; P{bs-GAL4.Term}G1 Wing disc
8 25754 P{UAS-Dcr-2.D}1; P{GawB}nubbin-AC-62 Wing disc
9 25755 P{UAS-Dcr-2.D}1; P{GawB}salm[LP39] Band of cells in the wing disc
10 25756 P{UAS-Dcr-2.D}1; P{GAL4-Mef2.R}R1 Somatic muscle cells
11 25757 P{UAS-Dcr-2.D}1; P{GawB}bbg[C96] Wing disc cells at the dorsal-ventral margin
12 25758 P{UAS-Dcr-2.D}1; P{GawB}pnr[MD237] Dorsal cells along length of the fly