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Split transcriptional activators
Stocks carrying "split" transcriptional activators are listed here. These stocks carry either 1) a construct that expresses a DNA-binding domain protein based on either GAL4 or lexA or 2) a construct that expresses a transcriptional activation domain based on either GAL4, VP16 or p65. These proteins also carry a common leucine zipper motif dimerization domain enabling a DNA-binding protein and an activation domain protein to interact. Expression of each construct may be driven by different enhancers and promoters with transcriptional activation only occuring in cells where both proteins are expressed and able to interact. The Drosophila "split GAL4" system was first described in Luan et al., 2006.

For stocks expressing both activation and DNA-binding domains, see:
- Janelia split GAL4 combination stocks
- Split transcription combination stocks (non-Janelia)
- Transcription-based calcium sensors

Go back to the main GAL4 page.
Stk # Promoter Symbol Genotype
      GAL4 DNA-binding domain stocks
60296 alphaTub84B P{Tub-GAL4DBD.D}2 y[*] w[*] P{w[+mC]=UAS-2xEYFP}AX; P{w[+mC]=Tub-GAL4DBD.D}2; Dr[1]/TM3, Sb[1]
60297 alphaTub84B P{Tub-GAL4DBD.D}3 y[*] w[*] P{w[+mC]=UAS-2xEYFP}AX; wg[Sp-1]/CyO; P{w[+mC]=Tub-GAL4DBD.D}3
60298 alphaTub84B P{Tub-GAL4DBD.D}2 y[1] w[*]; P{w[+mC]=Tub-GAL4DBD.D}2; P{w[+mC]=UAS-2xEGFP}AH3
60335 cac Mi{Trojan-GAL4DBD.0}cac[MI02836-TG4DBD.0] y[1] w[*] Mi{Trojan-GAL4DBD.0}cac[MI02836-TG4DBD.0]/FM7a
23869 ChAT P{ChAT-GAL4.DBD}J8A1 w[*]; P{w[+mC]=UAS-2xEGFP}AH2; P{w[+mC]=ChAT-GAL4.DBD}J8A1
60318 ChAT Mi{Trojan-GAL4DBD.0}ChAT[MI04508-TG4DBD.0] w[*]; l(2)*[*]/CyO; Mi{Trojan-GAL4DBD.0}ChAT[MI04508-TG4DBD.0]/TM3, Sb[1]
23867 elav P{elav-GAL4.DBD}H4A1 w[*]; P{w[+mC]=UAS-2xEGFP}AH2; P{w[+mC]=elav-GAL4.DBD}H4A1
61663 nSyb P{nSyb-MKII::GAL4DBDo}attP24 w[*]; P{y[+t7.7] w[+mC]=nSyb-MKII::GAL4DBDo}attP24/CyO; Pri[1]/TM6B
61662 nSyb P{nSyb-M13::GAL4DBDo}attP24 w[*]; P{y[+t7.7] w[+mC]=nSyb-M13::GAL4DBDo}attP24/CyO; Pri[1]/TM6B, Tb[1]
61664 nSyb P{nSyb-MKIIK11A::GAL4DBDo}attP24 w[*]; P{y[+t7.7] w[+mC]=nSyb-MKIIK11A::GAL4DBDo}attP24/CyO; Pri[1]/TM6B, Tb[1]
60313 VGlut Mi{Trojan-GAL4DBD.2}VGlut[MI04979-TG4DBD.2] w[*]; Mi{Trojan-GAL4DBD.2}VGlut[MI04979-TG4DBD.2]/CyO; MKRS/TM6B, Tb[1]
      lexA DNA-binding domain stocks
61665 nSyb P{nSyb-MKII::nlsLexADBDo}attP24 w[*]; P{y[+t7.7] w[+mC]=nSyb-MKII::nlsLexADBDo}attP24/CyO; Pri[1]/TM6B
61666 nSyb P{nSyb-MKIIK11A::nlsLexADBDo}attP24 w[*]; P{y[+t7.7] w[+mC]=nSyb-MKIIK11A::nlsLexADBDo}attP24/CyO; Pri[1]/TM6B, Tb[1]
56528 UAS P{UAS-lexA.DBD}2 y[1] w[*]; P{w[+mC]=UAS-lexA.DBD}2; TM2/TM6B, Tb[1]
56529 UAS P{UAS-lexA.DBD}3 y[1] w[*]; wg[Sp-1]/CyO; P{w[+mC]=UAS-lexA.DBD}3/TM6B, Tb[1]
61667 UAS P{UAS-MKII::nlsLexADBDo}attP40 w[*]; P{y[+t7.7] w[+mC]=UAS-MKII::nlsLexADBDo}attP40/CyO; Pri[1]/TM6B, Tb[1]
61668 UAS PBac{UAS-MKII::nlsLexADBDo}VK00005 w[*]; S[1]/CyO; PBac{y[+mDint2] w[+mC]=UAS-MKII::nlsLexADBDo}VK00005
      GAL4 transcriptional activation domain stocks
23868 elav P{elav-GAL4.AD}I1A1 w[*]; P{w[+mC]=UAS-2xEGFP}AH2; P{w[+mC]=elav-GAL4.AD}I1A1
23871 P{ET.GAL4.AD}O8 P{w[+mC]=ET.GAL4.AD}O8, w[*]; Pin[Yt]/CyO
      VP16 transcriptional activation domain stocks
60294 alphaTub84B P{Tub-dVP16AD.D}1 y[1] w[*] P{w[+mC]=UAS-2xEYFP}AX, P{w[+mC]=Tub-dVP16AD.D}1; wg[Sp-1]/CyO; Dr[1]/TM3, Sb[1]
60295 alphaTub84B P{Tub-dVP16AD.D}2 y[1] w[*]; P{w[+mC]=Tub-dVP16AD.D}2, P{w[+mC]=UAS-2xEGFP}AH2; Dr[1]/TM3, Sb[1]
23872 elav P{elav-VP16.AD}G3A1 w[*]; P{w[+mC]=UAS-2xEGFP}AH2; P{w[+mC]=elav-VP16.AD}G3A1
56526 ort P{ort-VP16.C2b.AD}2 P{ry[+t7.2]=hsFLP}22, y[1] w[*]; P{w[+mC]=ort-VP16.C2b.AD}2; TM2/TM6B, Tb[1]
60326 Shaw Mi{Trojan-dVP16AD.1}Shaw[MI01735-TdVP16AD.1] y[1] w[*]; Mi{Trojan-dVP16AD.1}Shaw[MI01735-TdVP16AD.1]
23870 P{ET.VP16.AD}N9 P{w[+mC]=ET.VP16.AD}N9, w[*]
56503 P{ET.VP16.AD}A609 w[*]; P{w[+mC]=ET.VP16.AD}A609
      p65 transcriptional activation domain stocks
60330 amon Mi{Trojan-p65AD.0}amon[MI00899-Tp65AD.0] y[1] w[*]; Mi{Trojan-p65AD.0}amon[MI00899-Tp65AD.0]/TM3, Sb[1]
60322 Gad1 Mi{Trojan-p65AD.2}Gad1[MI09277-Tp65AD.2] y[1] w[*]; wg[Sp-1]/CyO; Mi{Trojan-p65AD.2}Gad1[MI09277-Tp65AD.2]/TM3, Sb[1]
61811 QUAS P{QUAS-p65AD::CaM}3 w[*]; S[1]/CyO; P{w[+mC]=QUAS-p65AD::CaM}3/TM6B, Tb[1]
62566 QUAS P{QUAS-p65AD::CaM}2 w[*]; P{w[+mC]=QUAS-p65AD::CaM}2/CyO; Pri[1]/TM6B, Tb[1]
60329 Rdl Mi{Trojan-p65AD.1}Rdl[MI02957-Tp65AD.1] y[1] w[*]; wg[Sp-1]/CyO; Mi{Trojan-p65AD.1}Rdl[MI02957-Tp65AD.1]/TM3, Sb[1]
60327 Shaw Mi{Trojan-p65AD.1}Shaw[MI01735-Tp65AD.1] y[1] w[*]; Mi{Trojan-p65AD.1}Shaw[MI01735-Tp65AD.1]
61661 UAS M{UAS-p65AD::CaM}ZH-86Fb w[*]; S[1]/CyO; M{UAS-p65AD::CaM}ZH-86Fb
61669 UAS M{UAS-p65AD::CaM}ZH-86Fb w[*]; S[1]/CyO; M{w[+mC]=UAS-p65AD::CaM}ZH-86Fb, P{w[+mC]=tubP-GAL80[ts]}7