Stock Center logo
TRiP-CRISPR Toolbox stocks

The Transgenic RNAi Project (TRiP) is producing guide RNA transgenes for both overexpression and knockout of genes via CRISPR/Cas9 mechanisms. The stocks listed below were made by TRiP to facilitate use of these stocks.

P{UAS-3xFLAG.dCas9.VPR} expresses a nuclease-dead Cas9 fused to a chimeric activation domain under the control of UAS. It can be used to activate transcription of a gene in the presence of a guide RNA designed to facilitate overexpression of that gene. For more details see the TRiP-CRISPR overexpression site. The stocks below pair UAS-Cas9.VPR with various GAL4 and/or GAL80 insertions.

P{UAS-Cas9.P2} expresses Cas9 under the control of UAS. It can be used to create small insertions or deletions in a gene in a tissue-specific manner or in the germline in the presence of a guide RNA. For more details see the TRiP-CRISPR knockout site. The stocks below pair UAS-Cas9 with various GAL4 and/or GAL80 insertions.

See guide RNA stocks at Bloomington.

  Cas9 GAL4 GAL80 Stk # Genotype
1 control - expresses gRNA for QUAS 67539 y[1] sc[*] v[1]; P{y[+t7.7] v[+t1.8]=GS00089}attP40
2 P{UAS-3xFLAG.dCas9.VPR}attP2 P{bs-GAL4.Term}G1 67051 w[*]; P{w[+mC]=bs-GAL4.Term}G1/CyO; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP2
3 P{UAS-3xFLAG.dCas9.VPR}attP2 P{bs-GAL4.Term}G1 P{tubP-GAL80[ts]}2 67071 w[*]; P{w[+mC]=bs-GAL4.Term}G1/CyO; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP2, P{w[+mC]=tubP-GAL80[ts]}2
4 P{UAS-3xFLAG.dCas9.VPR}attP2 P{en2.4-GAL4}e16E 67049 w[*]; P{w[+mW.hs]=en2.4-GAL4}e16E, P{w[+mC]=UAS-2xEGFP}AH2/CyO; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP2
5 P{UAS-3xFLAG.dCas9.VPR}attP2 P{en2.4-GAL4}e16E P{tubP-GAL80[ts]}2 67069 w[*]; P{w[+mW.hs]=en2.4-GAL4}e16E, P{w[+mC]=UAS-2xEGFP}AH2/CyO; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP2, P{w[+mC]=tubP-GAL80[ts]}2
6 P{UAS-3xFLAG.dCas9.VPR}attP2 P{GAL4-nos.NGT}40 67052 w[*]; P{w[+mC]=GAL4-nos.NGT}40/CyO; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP2
7 P{UAS-3xFLAG.dCas9.VPR}attP2 P{GAL4-twi.2xPE}1 67053 w[*]; P{w[+mC]=GAL4-twi.2xPE}1/CyO; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP2
8 P{UAS-3xFLAG.dCas9.VPR}attP2 P{GawB}Myo31DF[NP0001] 67057 w[*]; P{w[+mW.hs]=GawB}Myo31DF[NP0001]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP2
9 P{UAS-3xFLAG.dCas9.VPR}attP2 P{GawB}Myo31DF[NP0001] P{tubP-GAL80[ts]}2 67067 w[*]; P{w[+mW.hs]=GawB}Myo31DF[NP0001]/CyO; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP2, P{w[+mC]=tubP-GAL80[ts]}2
10 P{UAS-3xFLAG.dCas9.VPR}attP2 P{GawB}NP5130 67054 w[*]; P{w[+mW.hs]=GawB}NP5130 P{w[+*]=UAS-GFP.U}2/CyO; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP2
11 P{UAS-3xFLAG.dCas9.VPR}attP2 P{GawB}NP5130 P{tubP-GAL80[ts]}2 67072 w[*]; P{w[+mW.hs]=GawB}NP5130 P{w[+*]=UAS-GFP.U}2; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP2, P{w[+mC]=tubP-GAL80[ts]}2
12 P{UAS-3xFLAG.dCas9.VPR}attP2 P{GawB}nubbin-AC-62 67055 w[*]; P{w[+mW.hs]=GawB}nubbin-AC-62; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP2
13 P{UAS-3xFLAG.dCas9.VPR}attP2 P{GawB}salm[LP39] 67050 w[*]; P{w[+mW.hs]=GawB}salm[LP39]/CyO; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP2
14 P{UAS-3xFLAG.dCas9.VPR}attP2 P{GawB}salm[LP39] P{tubP-GAL80[ts]}2 67070 w[*]; P{w[+mW.hs]=GawB}salm[LP39]/CyO; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP2, P{w[+mC]=tubP-GAL80[ts]}2
15 P{UAS-3xFLAG.dCas9.VPR}attP40 P{GAL4-dpp.blk1}40C.6 67045 w[*]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40/CyO; P{w[+mW.hs]=GAL4-dpp.blk1}40C.6/TM6B, Tb[1]
16 P{UAS-3xFLAG.dCas9.VPR}attP40 P{GAL4-dpp.blk1}40C.6 P{tubP-GAL80[ts]}10 67066 w[*]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40, P{w[+mC]=tubP-GAL80[ts]}10/CyO; P{w[+mW.hs]=GAL4-dpp.blk1}40C.6/TM6B, Tb[1]
17 P{UAS-3xFLAG.dCas9.VPR}attP40 P{GAL4-Mef2.R}R1 67041 w[*]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40/CyO; P{w[+mC]=GAL4-Mef2.R}R1
18 P{UAS-3xFLAG.dCas9.VPR}attP40 P{GAL4-Mef2.R}R1 P{tubP-GAL80[ts]}10 67063 w[*]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40, P{w[+mC]=tubP-GAL80[ts]}10; P{w[+mC]=GAL4-Mef2.R}R1
19 P{UAS-3xFLAG.dCas9.VPR}attP40 P{GAL4-Mhc.W}MHC-82 67044 w[*]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40; P{GAL4-Mhc.W}MHC-82/TM6B, Tb[1]
20 P{UAS-3xFLAG.dCas9.VPR}attP40 P{GAL4-Mhc.W}MHC-82 P{tubP-GAL80[ts]}10 67062 w[*]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40, P{w[+mC]=tubP-GAL80[ts]}10; P{GAL4-Mhc.W}MHC-82
21 P{UAS-3xFLAG.dCas9.VPR}attP40 P{GAL4}hh[Gal4] 67046 w[*]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40/CyO; P{w[+mC]=GAL4}hh[Gal4]/TM6B, Tb[1]
22 P{UAS-3xFLAG.dCas9.VPR}attP40 P{GawB}bbg[C96] 67042 w[*]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40/CyO; P{w[+mW.hs]=GawB}bbg[C96]
23 P{UAS-3xFLAG.dCas9.VPR}attP40 P{GawB}bbg[C96] P{tubP-GAL80[ts]}10 67064 w[*]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40, P{w[+mC]=tubP-GAL80[ts]}10; P{w[+mW.hs]=GawB}bbg[C96]
24 P{UAS-3xFLAG.dCas9.VPR}attP40 P{GawB}Bx[MS1096] 67039 w[1118] P{w[+mW.hs]=GawB}Bx[MS1096]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40/CyO
25 P{UAS-3xFLAG.dCas9.VPR}attP40 P{GawB}Bx[MS1096] P{tubP-GAL80[ts]}10 67059 w[1118] P{w[+mW.hs]=GawB}Bx[MS1096]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40, P{w[+mC]=tubP-GAL80[ts]}10
26 P{UAS-3xFLAG.dCas9.VPR}attP40 P{GawB}Dl[05151-G] P{tubP-GAL80[ts]}2 67047 w[*]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40/CyO; P{w[+mW.hs]=GawB}Dl[05151-G], P{w[+mC]=tubP-GAL80[ts]}2/TM6B, Tb[1]
27 P{UAS-3xFLAG.dCas9.VPR}attP40 P{GawB}elav[C155] P{tubP-GAL80[ts]}10 67058 P{w[+mW.hs]=GawB}elav[C155]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40, P{w[+mC]=tubP-GAL80[ts]}10
28 P{UAS-3xFLAG.dCas9.VPR}attP40 P{GawB}pnr[MD237] 67040 w[*]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40/CyO; P{w[+mW.hs]=GawB}pnr[MD237]/TM6B, Tb[1]
29 P{UAS-3xFLAG.dCas9.VPR}attP40 P{GawB}pnr[MD237] P{tubP-GAL80[ts]}10 67060 w[*]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40, P{w[+mC]=tubP-GAL80[ts]}10/CyO; P{w[+mW.hs]=GawB}pnr[MD237]/TM6B, Tb[1]
30 P{UAS-3xFLAG.dCas9.VPR}attP40 P{Lpp-GAL4.B}3 67043 w[*]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40/CyO; P{w[+mC]=Lpp-GAL4.B}3
31 P{UAS-3xFLAG.dCas9.VPR}attP40 P{Lpp-GAL4.B}3 P{tubP-GAL80[ts]}10 67061 w[*]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40, P{w[+mC]=tubP-GAL80[ts]}10; P{w[+mC]=Lpp-GAL4.B}3/TM6B, Tb[1]
32 P{UAS-3xFLAG.dCas9.VPR}attP40 P{tubP-GAL4}LL7 67048 w[*]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40; P{w[+mC]=tubP-GAL4}LL7/T(2;3)TSTL14, SM5: TM6B, Tb[1]
33 P{UAS-3xFLAG.dCas9.VPR}attP40 P{tubP-GAL4}LL7 P{tubP-GAL80[ts]}10 67065 w[*]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40, P{w[+mC]=tubP-GAL80[ts]}10; P{w[+mC]=tubP-GAL4}LL7/TM6B, Tb[1]
34 P{UAS-Cas9.P2}attP2 P{3.1Lsp2-Gal4}a 67087 w[*]; P{w[+mC]=3.1Lsp2-Gal4}a; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2/TM6B, Tb[1]
35 P{UAS-Cas9.P2}attP2 P{bs-GAL4.Term}G1 67082 w[*]; P{w[+mC]=bs-GAL4.Term}G1/CyO; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2/TM6B, Tb[1]
36 P{UAS-Cas9.P2}attP2 P{en2.4-GAL4}e16E 67080 w[*]; P{w[+mW.hs]=en2.4-GAL4}e16E, P{w[+mC]=UAS-2xEGFP}AH2/CyO; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2
37 P{UAS-Cas9.P2}attP2 P{GAL4-nos.NGT}40 67083 w[*]; P{w[+mC]=GAL4-nos.NGT}40; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2
38 P{UAS-Cas9.P2}attP2 P{GAL4-twi.2xPE}1 67084 w[*]; P{w[+mC]=GAL4-twi.2xPE}1/CyO; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2/TM6B, Tb[1]
39 P{UAS-Cas9.P2}attP2 P{GawB}Myo31DF[NP0001] 67088 w[*]; P{w[+mW.hs]=GawB}Myo31DF[NP0001]/CyO; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2/TM6B, Tb[1]
40 P{UAS-Cas9.P2}attP2 P{GawB}NP5130 67085 w[*]; P{w[+mW.hs]=GawB}NP5130 P{w[+*]=UAS-GFP.U}2/CyO; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2
41 P{UAS-Cas9.P2}attP2 P{GawB}nubbin-AC-62 67086 w[*]; P{w[+mW.hs]=GawB}nubbin-AC-62; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2
42 P{UAS-Cas9.P2}attP2 P{GawB}salm[LP39] 67081 w[*]; P{w[+mW.hs]=GawB}salm[LP39]/CyO; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2/TM6B, Tb[1]
43 P{UAS-Cas9.P2}attP40 P{GAL4-Mef2.R}R1 67075 w[*]; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP40/CyO; P{w[+mC]=GAL4-Mef2.R}R1/TM6B, Tb[1]
44 P{UAS-Cas9.P2}attP40 P{GAL4-Mhc.W}MHC-82 67079 w[*]; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP40/CyO; P{GAL4-Mhc.W}MHC-82/TM6B, Tb[1]
45 P{UAS-Cas9.P2}attP40 P{GawB}bbg[C96] 67076 w[*]; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP40/CyO; P{w[+mW.hs]=GawB}bbg[C96]
46 P{UAS-Cas9.P2}attP40 P{GawB}Bx[MS1096] 67074 w[1118] P{w[+mW.hs]=GawB}Bx[MS1096]; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP40/CyO
47 P{UAS-Cas9.P2}attP40 P{GawB}elav[C155] 67073 P{w[+mW.hs]=GawB}elav[C155]; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP40/CyO
48 P{UAS-Cas9.P2}attP40 P{GawB}pnr[MD237] 67077 w[*]; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP40/CyO; P{w[+mW.hs]=GawB}pnr[MD237]/TM6B, Tb[1]
49 P{UAS-Cas9.P2}attP40 P{Lpp-GAL4.B}3 67078 w[*]; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP40/CyO; P{w[+mC]=Lpp-GAL4.B}3/TM6B, Tb[1]