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guide RNA stocks

All available TRiP-CRISPR guide RNA lines are listed here. The insertions must be combined with a source of Cas9 to induce gene knockout or overexpression. See the TRiP-CRISPR toolbox for a list of available stocks with both Cas9 and a GAL4 driver.

There are two TRiP-CRISPR gRNA stock collections (for specifics, please see the TRiP in vivo CRISPR site):

  1. TRiP-CRISPR Overexpression (TRiP-OE) - activate expression of the target gene in somatic and/or germline cells by crossing to a line expressing GAL4 and dCas9-VPR
  2. TRiP-CRISPR KnockOut (TRiP-KO) - generate indels (small insertion or deletion mutations) in somatic and/or germline cells by crossing to a line expressing GAL4 and Cas9

TRiP-CRISPR lines are generated in either pCFD3 or pCFD4 vectors (Port et al., 2014) which are designed to ubiquitiously express either one or two sgRNAs, respectively. Both vectors contain attB sites for phiC31-recombination, as well as the vermillion selection marker. In pCFD3, a single sgRNA is driven by the snRNA:U6:96Ac (U6:3) promoter and in pCFD4 the sgRNAs are driven by snRNA:U6:96Aa (U6:1) and snRNA:U6:96Ac. Detailed descriptions of these vectors are available at the CRISPR Fly Design site.

The TRiP encourages the community to nominate genes for TRiP-OE and TRiP-KO production via the TRiP sgRNA Database. If use of any of the TRiP-CRISPR stocks results in data used in a publication, please cite the TRiP in your publication.

** If indicated, this insertion targets more than one gene. To see the other targets, either search this page with the stock number or click on the stock number to read the stock report.

  Assoc. Gene Multiple Targets** Vector Symbol Chr. Stk #
1 Acon pCFD4 P{TOE.GS00476}attP40 2 68079
2 Acox57D-d pCFD4 P{TOE.GS00477}attP40 2 68080
3 Acox57D-p pCFD4 P{TOE.GS00478}attP40 2 68081
4 Actbeta pCFD4 P{TOE.GS00028}attP40 2 67641
5 Ady43A pCFD4 P{TOE.GS00479}attP40 2 68082
6 Alas pCFD4 P{TOE.GS00480}attP40 2 68083
7 Aldh pCFD4 P{TOE.GS00481}attP40 2 68084
8 alpha-Man-Ib multiple targets pCFD4 P{TOE.GS00397}attP40 2 67602
9 aos pCFD4 P{TOE.GS00057}attP40 2 67531
10 ATPsynepsilonL pCFD3 P{TKO.GS00432}attP40 2 68048
11 ATPsynepsilonL pCFD4 P{TOE.GS00352}attP40 2 67595
12 bc10 pCFD4 P{TOE.GS00359}attP40 2 67596
13 bgm pCFD4 P{TOE.GS00482}attP40 2 68085
14 bnl pCFD4 P{TOE.GS00107}attP40 2 67542
15 cathD pCFD4 P{TOE.GS00483}attP40 2 68086
16 CG10924 pCFD4 P{TOE.GS00484}attP40 2 68087
17 CG11426 pCFD4 P{TOE.GS00485}attP40 2 68088
18 CG11438 pCFD4 P{TOE.GS00486}attP40 2 68089
19 CG11876 multiple targets pCFD4 P{TOE.GS00397}attP40 2 67602
20 CG11897 pCFD4 P{TOE.GS00540}attP40 2 68143
21 CG11897 pCFD4 P{TOE.GS00541}attP40 2 68144
22 CG12384 pCFD3 P{TKO.GS00437}attP40 2 68052
23 CG12384 pCFD4 P{TOE.GS00293}attP40 2 67577
24 CG12746 pCFD4 P{TOE.GS00487}attP40 2 68090
25 CG1315 pCFD4 P{TOE.GS00488}attP40 2 68091
26 CG13252 pCFD4 P{TOE.GS00195}attP40 2 67561
27 CG14483 pCFD4 P{TOE.GS00267}attP40 2 68014
28 CG1461 pCFD4 P{TOE.GS00489}attP40 2 68092
29 CG14812 pCFD4 P{TOE.GS00336}attP40 2 67590
30 CG14818 pCFD4 P{TOE.GS00288}attP40 2 67576
31 CG14933 pCFD4 P{TOE.GS00298}attP40 2 68023
32 CG14967 pCFD4 P{TOE.GS00185}attP40 2 67557
33 CG1504 pCFD4 P{TOE.GS00075}attP40 2 67534
34 CG15450 pCFD4 P{TOE.GS00490}attP40 2 68093
35 CG15456 pCFD4 P{TOE.GS00265}attP40 2 67566
36 CG15531 pCFD4 P{TOE.GS00491}attP40 2 68094
37 CG15635 pCFD4 P{TOE.GS00015}attP40 2 67518
38 CG15829 pCFD4 P{TOE.GS00282}attP40 2 67573
39 CG16713 pCFD4 P{TOE.GS00270}attP40 2 68015
40 CG17027 pCFD4 P{TOE.GS00492}attP40 2 68095
41 CG17028 pCFD4 P{TOE.GS00493}attP40 2 68096
42 CG17283 pCFD4 P{TOE.GS00494}attP40 2 68097
43 CG17544 pCFD4 P{TOE.GS00495}attP40 2 68098
44 CG17691 pCFD4 P{TOE.GS00496}attP40 2 68099
45 CG17734 pCFD4 P{TOE.GS00261}attP40 2 68012
46 CG17807 pCFD3 P{TKO.GS00412}attP40 2 67605
47 CG18012 pCFD4 P{TOE.GS00346}attP40 2 67593
48 CG18508 pCFD4 P{TOE.GS00317}attP40 2 68026
49 CG18563 pCFD4 P{TOE.GS00082}attP40 2 67536
50 CG2137 pCFD4 P{TOE.GS00741}attP40 2 68145
51 CG2201 pCFD4 P{TOE.GS00435}attP40 2 68051
52 CG2816 pCFD4 P{TOE.GS00285}attP40 2 68019
53 CG30103 pCFD4 P{TOE.GS00497}attP40 2 68100
54 CG30120 multiple targets pCFD4 P{TOE.GS00392}attP40 2 67599
55 CG31075 pCFD4 P{TOE.GS00498}attP40 2 68101
56 CG31468 pCFD4 P{TOE.GS00326}attP40 2 68029
57 CG31473 pCFD4 P{TOE.GS00499}attP40 2 68102
58 CG3209 pCFD4 P{TOE.GS00500}attP40 2 68103
59 CG32448 pCFD4 P{TOE.GS00334}attP40 2 67588
60 CG32698 pCFD4 P{TOE.GS00093}attP40 2 67540
61 CG32736 pCFD4 P{TOE.GS00272}attP40 2 67568
62 CG33096 pCFD4 P{TOE.GS00002}attP40 2 67517
63 CG34172 pCFD4 P{TOE.GS00335}attP40 2 67589
64 CG34228 pCFD4 P{TOE.GS00318}attP40 2 67584
65 CG34229 pCFD4 P{TOE.GS00353}attP40 2 68032
66 CG34263 pCFD4 P{TOE.GS00275}attP40 2 67570
67 CG34276 pCFD4 P{TOE.GS00273}attP40 2 68016
68 CG3566 pCFD4 P{TOE.GS00356}attP40 2 68033
69 CG3809 pCFD4 P{TOE.GS00503}attP40 2 68106
70 CG40228 pCFD4 P{TOE.GS00295}attP40 2 68022
71 CG40472 pCFD4 P{TOE.GS00296}attP40 2 67578
72 CG4101 pCFD3 P{TKO.GS00455}attP40 2 68064
73 CG42259 pCFD4 P{TOE.GS00280}attP40 2 68018
74 CG42308 pCFD4 P{TOE.GS00314}attP40 2 67583
75 CG42375 pCFD4 P{TOE.GS00271}attP40 2 67567
76 CG42456 pCFD4 P{TOE.GS00284}attP40 2 67575
77 CG42460 pCFD4 P{TOE.GS00283}attP40 2 67574
78 CG42464 pCFD4 P{TOE.GS00278}attP40 2 67571
79 CG42486 pCFD4 P{TOE.GS00289}attP40 2 68020
80 CG42496 pCFD4 P{TOE.GS00266}attP40 2 68013
81 CG42538 pCFD3 P{TKO.GS00439}attP40 2 68054
82 CG42566 pCFD4 P{TOE.GS00333}attP40 2 67587
83 CG42717 pCFD4 P{TOE.GS00304}attP40 2 68025
84 CG42787 pCFD4 P{TOE.GS00308}attP40 2 67581
85 CG4302 pCFD4 P{TOE.GS00504}attP40 2 68107
86 CG43069 pCFD4 P{TOE.GS00381}attP40 2 67598
87 CG43229 pCFD4 P{TOE.GS00372}attP40 2 67597
88 CG43324 pCFD4 P{TOE.GS00305}attP40 2 67580
89 CG43350 pCFD4 P{TOE.GS00347}attP40 2 67594
90 CG44194 pCFD4 P{TOE.GS00301}attP40 2 68024
91 CG44296 pCFD4 P{TOE.GS00321}attP40 2 68027
92 CG45011 pCFD4 P{TOE.GS00279}attP40 2 68017
93 CG4650 pCFD4 P{TOE.GS00350}attP40 2 68031
94 CG4660 pCFD4 P{TOE.GS00147}attP40 2 67549
95 CG4998 pCFD4 P{TOE.GS00154}attP40 2 67552
96 CG5009 pCFD4 P{TOE.GS00506}attP40 2 68109
97 CG5189 multiple targets pCFD4 P{TOE.GS00392}attP40 2 67599
98 CG5214 pCFD4 P{TOE.GS00507}attP40 2 68110
99 CG5493 pCFD4 P{TOE.GS00443}attP40 2 68058
100 CG5828 pCFD4 P{TOE.GS00509}attP40 2 68112
101 CG5966 pCFD4 P{TOE.GS00510}attP40 2 68113
102 CG6066 pCFD4 P{TOE.GS00400}attP40 2 67603
103 CG6115 pCFD4 P{TOE.GS00337}attP40 2 67591
104 CG6225 pCFD4 P{TOE.GS00440}attP40 2 68055
105 CG6244 pCFD4 P{TOE.GS00291}attP40 2 68021
106 CG6475 pCFD4 P{TOE.GS00511}attP40 2 68114
107 CG6508 pCFD4 P{TOE.GS00512}attP40 2 68115
108 CG6770 pCFD4 P{TOE.GS00361}attP40 2 68034
109 CG7140 pCFD4 P{TOE.GS00461}attP40 2 68070
110 CG7296 pCFD4 P{TOE.GS00188}attP40 2 67559
111 CG7358 pCFD3 P{TKO.GS00449}attP40 2 68063
112 CG7637 pCFD4 P{TOE.GS00274}attP40 2 67569
113 CG7768 pCFD4 P{TOE.GS00513}attP40 2 68116
114 CG8036 pCFD4 P{TOE.GS00514}attP40 2 68117
115 CG8199 pCFD4 P{TOE.GS00515}attP40 2 68118
116 CG8311 pCFD4 P{TOE.GS00393}attP40 2 68037
117 CG8379 multiple targets pCFD4 P{TOE.GS00395}attP40 2 67600
118 CG8498 pCFD4 P{TOE.GS00404}attP40 2 67604
119 CG8629 pCFD4 P{TOE.GS00325}attP40 2 68028
120 CG8630 pCFD4 P{TOE.GS00516}attP40 2 68119
121 CG9065 pCFD4 P{TOE.GS00327}attP40 2 67585
122 CG9265 pCFD4 P{TOE.GS00517}attP40 2 68120
123 CG9527 pCFD4 P{TOE.GS00441}attP40 2 68056
124 CG9669 pCFD4 P{TOE.GS00281}attP40 2 67572
125 CG9743 pCFD4 P{TOE.GS00744}attP40 2 68148
126 ChAT pCFD4 P{TOE.GS00463}attP40 2 68072
127 cN-IIIB pCFD4 P{TOE.GS00502}attP40 2 68105
128 Coprox pCFD4 P{TOE.GS00521}attP40 2 68124
129 COX6AL pCFD4 P{TOE.GS00263}attP40 2 67565
130 ct pCFD4 P{TOE.GS00041}attP40 2 67524
131 Cyp1 pCFD4 P{TOE.GS00522}attP40 2 68125
132 Cyp18a1 pCFD4 P{TOE.GS00742}attP40 2 68146
133 Cyp9b1 pCFD4 P{TOE.GS00523}attP40 2 68126
134 Cyp9f2 pCFD4 P{TOE.GS00442}attP40 2 68057
135 daw pCFD4 P{TOE.GS00051}attP40 2 67529
136 Dhc36C multiple targets pCFD4 P{TOE.GS00396}attP40 2 67601
137 dnk pCFD4 P{TOE.GS00524}attP40 2 68127
138 dpp pCFD4 P{TOE.GS00163}attP40 2 67554
139 egr pCFD4 P{TOE.GS00111}attP40 2 67543
140 Eip55E pCFD4 P{TOE.GS00525}attP40 2 68128
141 fbl pCFD4 P{TOE.GS00526}attP40 2 68129
142 fbp pCFD4 P{TOE.GS00527}attP40 2 68130
143 FK506-bp2 pCFD4 P{TOE.GS00528}attP40 2 68131
144 FKBP59 pCFD4 P{TOE.GS00529}attP40 2 68132
145 gbb pCFD4 P{TOE.GS00073}attP40 2 67533
146 Gclc pCFD3 P{TKO.GS00426}attP40 2 68045
147 Gclc pCFD4 P{TOE.GS00538}attP40 2 68141
148 Gclc pCFD4 P{TOE.GS00539}attP40 2 68142
149 GluRIIA pCFD3 P{TKO.GS00444}attP40 2 68059
150 GluRIIA pCFD4 P{TOE.GS00474}attP40 2 68078
151 GlyP pCFD4 P{TOE.GS00530}attP40 2 68133
152 Gpo-1 pCFD4 P{TOE.GS00531}attP40 2 68134
153 grk pCFD4 P{TOE.GS00045}attP40 2 67525
154 Gs2 pCFD4 P{TOE.GS00532}attP40 2 68135
155 GstE4 pCFD4 P{TOE.GS00533}attP40 2 68136
156 GstE5 pCFD4 P{TOE.GS00534}attP40 2 68137
157 GstE9 pCFD4 P{TOE.GS00464}attP40 2 68073
158 GstT2 pCFD4 P{TOE.GS00743}attP40 2 68147
159 GstT3 pCFD4 P{TOE.GS00465}attP40 2 68074
160 GstZ1 pCFD4 P{TOE.GS00466}attP40 2 68075
161 GstZ2 pCFD4 P{TOE.GS00467}attP40 2 68076
162 hh pCFD4 P{TOE.GS00191}attP40 2 67560
163 hll pCFD4 P{TOE.GS00505}attP40 2 68108
164 Hn pCFD4 P{TOE.GS00468}attP40 2 68077
165 HP1Lcsd pCFD4 P{TOE.GS00330}attP40 2 67586
166 IA-2 pCFD4 P{TOE.GS00251}attP40 2 68008
167 Irp-1A pCFD4 P{TOE.GS00420}attP40 2 68039
168 IscU multiple targets pCFD4 P{TOE.GS00395}attP40 2 67600
169 jeb pCFD4 P{TOE.GS00159}attP40 2 67553
170 Krn pCFD4 P{TOE.GS00186}attP40 2 67558
171 l(1)G0334 pCFD4 P{TOE.GS00446}attP40 2 68061
172 l(3)02640 pCFD4 P{TOE.GS00421}attP40 2 68040
173 lace pCFD4 P{TOE.GS00422}attP40 2 68041
174 laza pCFD4 P{TOE.GS00429}attP40 2 68046
175 LManI pCFD4 P{TOE.GS00508}attP40 2 68111
176 LManIII pCFD4 P{TOE.GS00518}attP40 2 68121
177 LManIV pCFD4 P{TOE.GS00519}attP40 2 68122
178 LManV pCFD4 P{TOE.GS00520}attP40 2 68123
179 LPCAT pCFD4 P{TOE.GS00501}attP40 2 68104
180 mav pCFD4 P{TOE.GS00018}attP40 2 67520
181 mEFTs multiple targets pCFD4 P{TOE.GS00396}attP40 2 67601
182 Membrin multiple targets pCFD4 P{TOE.GS00389}attP40 2 68036
183 Mgstl pCFD4 P{TOE.GS00430}attP40 2 68047
184 muc pCFD4 P{TOE.GS00462}attP40 2 68071
185 myo pCFD4 P{TOE.GS00047}attP40 2 67526
186 Nach pCFD3 P{TKO.GS00249}attP40 2 68006
187 Nach pCFD4 P{TOE.GS00257}attP40 2 67562
188 ND-39 pCFD4 P{TOE.GS00387}attP40 2 68035
189 ND-AGGG pCFD4 P{TOE.GS00345}attP40 2 67592
190 ND-MLRQ pCFD4 P{TOE.GS00297}attP40 2 67579
191 Nmnat pCFD4 P{TOE.GS00423}attP40 2 68042
192 peb pCFD4 P{TOE.GS00052}attP40 2 67530
193 ppk16 pCFD3 P{TKO.GS00252}attP40 2 68009
194 ppk16 pCFD4 P{TOE.GS00259}attP40 2 67564
195 ppk28 pCFD3 P{TKO.GS00410}attP40 2 68038
196 ppk6 pCFD3 P{TKO.GS00254}attP40 2 68010
197 ppk6 pCFD4 P{TOE.GS00258}attP40 2 67563
198 pps pCFD3 P{TKO.GS00445}attP40 2 68060
199 pr pCFD4 P{TOE.GS00456}attP40 2 68065
200 Pu pCFD4 P{TOE.GS00433}attP40 2 68049
201 Pvf1 pCFD4 P{TOE.GS00032}attP40 2 67522
202 Pvf2 pCFD4 P{TOE.GS00162}attP40 2 67634
203 Pvf3 pCFD4 P{TOE.GS00022}attP40 2 67521
204 pyr pCFD4 P{TOE.GS00085}attP40 2 67537
205 QUAS pCFD4 P{GS00089}attP40 2 67539
206 r pCFD4 P{TOE.GS00457}attP40 2 68066
207 RNASEK pCFD3 P{TKO.GS00438}attP40 2 68053
208 ry pCFD4 P{TOE.GS00458}attP40 2 68067
209 Sam-S pCFD4 P{TOE.GS00434}attP40 2 68050
210 scw pCFD4 P{TOE.GS00080}attP40 2 67535
211 skap pCFD4 P{TOE.GS00535}attP40 2 68138
212 SLIRP2 pCFD4 P{TOE.GS00332}attP40 2 68030
213 snf pCFD3 P{TKO.GS00425}attP40 2 68044
214 Spt-I pCFD4 P{TOE.GS00537}attP40 2 68140
215 spz pCFD4 P{TOE.GS00152}attP40 2 67551
216 spz3 pCFD4 P{TOE.GS00008}attP40 2 68005
217 spz4 pCFD4 P{TOE.GS00016}attP40 2 67519
218 spz5 pCFD4 P{TOE.GS00130}attP40 2 67547
219 spz6 pCFD4 P{TOE.GS00088}attP40 2 67538
220 ths pCFD4 P{TOE.GS00134}attP40 2 67635
221 Trh pCFD4 P{TOE.GS00459}attP40 2 68068
222 trk pCFD4 P{TOE.GS00062}attP40 2 67532
223 Uev1A multiple targets pCFD4 P{TOE.GS00389}attP40 2 68036
224 Ugt86De pCFD4 P{TOE.GS00460}attP40 2 68069
225 upd1 pCFD4 P{TOE.GS00169}attP40 2 67555
226 upd2 pCFD4 P{TOE.GS00171}attP40 2 67556
227 upd3 pCFD4 P{TOE.GS00129}attP40 2 67546
228 veil pCFD4 P{TOE.GS00424}attP40 2 68043
229 vn pCFD4 P{TOE.GS00144}attP40 2 67548
230 Vti1b pCFD4 P{TOE.GS00312}attP40 2 67582
231 wg pCFD4 P{TOE.GS00125}attP40 2 67545
232 whd pCFD4 P{TOE.GS00536}attP40 2 68139
233 Wnt10 pCFD4 P{TOE.GS00034}attP40 2 67523
234 Wnt2 pCFD4 P{TOE.GS00120}attP40 2 67544
235 Wnt4 pCFD4 P{TOE.GS00048}attP40 2 67527
236 Wnt5 pCFD4 P{TOE.GS00148}attP40 2 67550
237 Wnt6 pCFD4 P{TOE.GS00049}attP40 2 67528
238 wntD pCFD4 P{TOE.GS00104}attP40 2 67541
239 ZnT33D pCFD4 P{TOE.GS00255}attP40 2 68011
240 ZnT35C pCFD4 P{TOE.GS00250}attP40 2 68007
241 ZnT63C pCFD3 P{TKO.GS00447}attP40 2 68062